This study evaluated the effects of different dietary n-6/n-3 polyunsaturated fatty acid (PUFA) ratios on in vitro ruminant fermentation. Methane production, composition, and microbial profiles were compared after fermentation rumen fluid collected from cows that had been fed isoenergetic isoproteic experimental diets at three ratios: 3.04 (HN6, high n-6 source), 2.03 (MN6, medium 0.8 (LN6, low...