Copper efflux oxidases (CueOs) are key enzymes in copper homeostasis systems. The mechanisms involved however largely unknown. CueO-type share a typical structural feature composed of Methionine-rich (Met-rich) domains that proposed to be homeostasis. Bioelectrocatalysis using the presence Cu2+ recently highlighted new Cu2+-dependent catalytic pathway related cuprous oxidase activity. In this w...

Enzyme immobilization on solid conducting surfaces faces challenges for practical applications in technologies such as biosensors and biofuel cells. Short-term stability, poor electrochemical performance, enzyme inhibition are some unsolved issues. Here, we show a simple methodology bilirubin oxidase (BOD) carbon-based gas diffusion electrode four-electron oxygen reduction reaction. BOD is inco...