Berberine is an alkaloid found in plants such as goldenseal (Hydrastis Canadensis L.), European barberry or jaundice berry (Berberis vulgaris L.), and Chinese goldthread or Huanglian (Coptis chinensis Franch.). Goldenseal can be grown in Canada and natural health products derived from this plant have been found on consumer shelves. In this review, a UPLC-based analysis of berberine in a goldens...

Suppose one is given a (incomplete) specification of a set of interacting reactions. We would use this set of formulas as the background theory T . Suppose additionally, that a number of observations are made referring to reactions known to have occurred, or compounds known to be available for participation in a reaction, etc. Let α denote the formula representing these observations. Generally,...

The representation of a biochemical network as a graph is the coarsest level of description in cellular biochemistry. By studying the network structure one can draw conclusions on the large scale organisation of the biochemical processes. We describe methods how one can extract hierarchies of subnetworks, how these can be interpreted and further deconstructed to find autonomous subnetworks. The...

MOTIVATION Large biochemical networks pose a unique challenge from the point of view of evaluating conservation laws. The computational problem in most cases exceeds the capability of available software tools, often resulting in inaccurate computation of the number and form of conserved cycles. Such errors have profound effects on subsequent calculations, particularly in the evaluation of the J...

The paper applies MEKC technique to simultaneously carry out quantitative analysis on dairy product’s melamine (Mel) and 5-hydroxymethyl furfural (HMF) bi-component. The quantization analytic experiment starts with a systematic research on operating voltage, electrophoretic buffer solution gravity and optimum pH value, which further influences additives’ concentration as well as other factors, ...

We introduce a novel optofuidic biochemical sensor in which detection is based on the contrast between the refractive index of a target biomaterial and ambient carrier fluid. The sensor consists of parallel vertical micochannels embedded in a planar substrate. The central microchannel is illuminated by a focused beam of light and the periodic spacing of the channels combined with the contrast i...

We investigate how stochastic reaction processes are affected by external perturbations. We describe an extension of the deterministic metabolic control analysis (MCA) to the stochastic regime. We introduce stochastic sensitivities for mean and covariance values of reactant concentrations and reaction fluxes and show that there exist MCA-like summation theorems among these sensitivities. The su...

Sensitivity analysis is an indispensable tool for studying the robustness and fragility properties of biochemical reaction systems as well as for designing optimal approaches for selective perturbation and intervention. Deterministic sensitivity analysis techniques, using derivatives of the system response, have been extensively used in the literature. However, these techniques suffer from seve...

We propose a new framework for rigorous robustness analysis of stochastic biochemical systems that is based on probabilistic model checking techniques. We adapt the general definition of robustness introduced by Kitano to the class of stochastic systems modelled as continuous time Markov Chains in order to extensively analyse and compare robustness of biological models with uncertain parameters...

abstract   background: irregular working hours, including night work and shift work, have been found to be associated with alteration in various levels of biochemical factors. and some studies have showed association between shift work and blood biochemical disturbances in blood. in this epidemiological study we investigated, whether regular schedule of working hours can minimize the associated...