Isothermal titration calorimetry (ITC) is a popular chemical analysis technique that can be used to measure macromolecular interactions and physical processes. ITC involves the measurement of heat flow from cell after each injection during experiment. has been useful thermodynamics such as protein-ligand or protein-protein binding affinity also processes enzyme catalyzed reactions. The use in b...

Enzyme-CBM fusion constructs immobilised on wood-derived cellulose scaffolds: a sustainable approach for continuous flow biocatalysis.

Biocat collection http://www.biocatcollection.net/cms/ The Biocat collection is a repository of organisms and enzymes that are available for screening or other uses in biocatalysis. The collection includes oxidoreductases, hydrolases, lyases and polymerases. BRENDA http://www.brenda-enzymes.info/ BRENDA is a comprehensive database of enzymes organized with practical usage in mind. There is exte...

Biocatalysis entails the use of enzymes, which are catalytically active proteins, to speed up reactions so that products can be obtained swiftly and accurately [...]

An immense number of chemical reactions are carried out simultaneously in living cells. NatureÏs optimization approach encompasses the assembly of reactions in cascades and to embed them in finely tuned metabolic networks. With the vast progress in the field of biocatalysis, man-made cascades, especially redox cascades, have reached a degree of complexity that needs tools for improved control a...

The immobilisation of proteins on to nanoparticles has a number of applications ranging from biocatalysis through to cellular delivery of biopharmaceuticals. Here we describe a phosphopantetheinyl transferase (Sfp)-catalysed method for immobilising proteins bearing a small 12-mer "ybbR" tag on to nanoparticles functionalised with coenzyme A. The Sfp-catalysed immobilisation of proteins on to na...

Pseudomonas stutzeri SDM-LAC is an efficient lactate utilizer with various applications in biocatalysis. Here we present a 4.2-Mb assembly of its genome. The annotated four adjacent genes form a lactate utilization operon, which could provide further insights into the molecular mechanism of lactate utilization.

Multienzyme processes represent an important area of biocatalysis. Their efficiency can be enhanced by optimization of the stoichiometry of the biocatalysts. Here we present a workflow for maximizing the efficiency of a three-enzyme system catalyzing a five-step chemical conversion. Kinetic models of pathways with wild-type or engineered enzymes were built, and the enzyme stoichiometry of each ...