Methanolic extracts of Vitex negundo (Nochi), Coleus amboinicus (Karpuravalli), Hylocereus polyrhyzus (Dragon fruit) and Andrographis paniculata (Hempedu Bumi) were evaluated in an effort to identify antibacterial compounds against clinical isolates of Methicillin-Resistant Staphylococcus aureus (MRSA). Coleus amboinicus crude methanolic (CACM) leaves extract inhibited MRSA growth completely at...

Samples of maternal and foetal blood and of human milk, cow's milk, and dried cow's milk have been analysed for cobalamins by chromatography and bioautography. Plasma cobalamins in late pregnancy and after delivery differed quantitatively from those in healthy non-pregnant women. Differences in the proportions of plasma cobalamins were also observed between mothers and their newborn infants. Th...

Chemical fungicides are an important component in disease management for most crops. As part of a program to discover natural product-based fungicides, several sensitive assay systems have been developed for the evaluation of naturally occurring antifungal agents. In this study, we focused on the discovery and evaluation of new natural product-based fungicides from Angelica sinensis (Dong Quai)...

BACKGROUND Candida albicans has become resistant to the already limited, toxic and expensive anti-Candida agents available in the market. These factors necessitate the search for new anti-fungal agents. METHODS Sixty-three plant extracts, from 56 Tanzanian plant species obtained through the literature and interviews with traditional healers, were evaluated for anti-Candida activity. Aqueous m...

Different solvent extracts of a red algae, Hypnea flagelliformis, and two brown algae, Cystoseira myrica and Sargassum boveanum, which were collected from the Persian Gulf coast were subjected to different bioassays, including: 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, antibacterial and antifungal activity by thin layer chromatography (TLC)-bioautography, agar disc dif...

One-directional paper chromatography followed by bioautography has proved useful for differentiating antibiotics produced by Bacillus species in connection with a screening program. For purposes of comparison with unidentified antibiotics, Rf values have been determined with a number of solvents for most of the available Bacillus antibiotics active against gram positive bacteria and several of ...

Physalis minima is an important medicinal plant of Indian System of Medicine. This plant is reported for its diuretic, laxative and antiinflammatory activities. However, the plant is not well scrutinized for its antimicrobial potential. The major chemical constituents reported from the plant are phenolics and alkaloids, which suggest that the plant may turn out to be a potent antiinfective agen...

The production of acid in milk cultures of lactic streptococci was stimulated by the addition of corn steep liquor. Separation by ion-exchange and paper chromatography indicated the presence of four major stimulatory components in the corn steep. Some variation was noted in the response of the lactic streptococci to the individual stimulatory components. The four components were further purifie...

This entry describes applications of known indicators and dyes as new visualizing reagents and various visualizing systems as well as photocatalytic reactions and bioautography method for the detection of bioactive compounds including drugs and compounds isolated from herbal extracts. Broadening index, detection index, characteristics of densitometric band, modified contrast index, limit of det...

Two new garcinia acid derivatives, 2-(butoxycarbonylmethyl)-3-butoxycarbonyl-2-hydroxy-3-propanolide and 1',1"-dibutyl methyl hydroxycitrate, were isolated from the fruits of Garcinia atroviridis guided by TLC bioautography against the fungus Cladosporium herbarum. The structures of these compounds were established by spectral analysis. The former compound represents a unique beta-lactone struc...