The effects of phytase supplementation on the apparent ileal digestibility (AID) of amino acids (AA) have been inconsistent. Two experiments evaluated the effect of providing a mixture of pancreatic enzymes (Pancreatin(®) ) to growing pigs fed sorghum-soybean meal diets supplemented with phytase on the AID of AA, energy, and phosphorus (P), as well as the ileal digestibility (ID) of phytate; th...

The efficacy of a recombinantly derived microbial phytase (Natuphos 5000, BASF Corp.) was evaluated in sorghum-soybean meal-based diets of finishing swine. During the 50- to 80- and 80- to 118-kg BW intervals, diets contained .40 and .39% plant P, respectively; control diets fed during the two weight intervals were supplemented with .08 and .04% inorganic P from dicalcium phosphate. The all pla...

Efficacies of phytase, phosphorolytic enzymes (phytase + acid phosphatase), an enzymic "cocktail" (phytase + acid phosphatase + pectinase + citric acid), a novel Aspergillus niger (fungal) mycelium (FM), and FM enriched in phytase and antioxidants were investigated in growing broilers (Days 1 to 21) fed wheat-based diets. Broilers were fed the following seven diets at 0.69% Ca: 1) a negative co...

Forty-eight grower pigs were used to evaluate the effects of feeding low phytic acid (LPA) corn, LPA soybean meal, normal corn (NC), normal soybean meal (NSBM), and phytase on nutrient digestibility and excretion. Barrows were blocked by BW (initial BW=45.3+/-1.6 kg) and randomly assigned to 1 of 8 dietary treatments in a 2 x 2 x 2 factorial arrangement (6 pigs/treatment). Pigs were fed twice d...

Sixteen cannulated pigs were used to evaluate the effect of a new 6-phytase derived from Buttiauxella spp. and expressed in Trichoderma reesei on apparent ileal digestibility (AID) of AA and apparent total tract digestibility (ATTD) of DM, N, Ca, P, Na, Mg, K, Cl, and energy. Pigs were fed 4 diets for 2 periods in a crossover design. Within each period, there were 4 blocks of 4 pigs per block w...

Background: Degradation of phytic acid to inorganic phosphate in domestic animals’ diets requires thermostable phytase. Although Basillus subtilis phytase shows a potential to be degraded phytate complex in high temperature, the enzyme activities and yields need to be increased to make them possible for industrial application. Methods: The phytase gene from Bacillus subtilis DR8886 was...

Previously, we determined the DNA and amino acid sequences as well as biochemical and biophysical properties of a series of fungal phytases. The amino acid sequences displayed 49-68% identity between species, and the catalytic properties differed widely in terms of specific activity, substrate specificity, and pH optima. With the ultimate goal to combine the most favorable properties of all phy...

Forty-eight pigs (barrows:gilts, 1:1) with an average initial weight of 9.4 kg were used in a 2 x 2 factorial experiment to determine the influence of dietary phytase (0 or 1,500 phytase units/kg) and zinc (0 or 100 mg/kg) supplementation of a corn-soybean meal diet on the utilization of P, Ca, Cu, Mg, Mn, and Zn. After a 21-d growth experiment, feed was withheld for 24 h and blood was collecte...

A study was conducted to determine the additive effects of calcium (Ca) levels and the enzyme phytase, organic acids (citric, ascorbic acid), vitamin D on broiler performance and nutrient digestibility (d 3 1 to 21) in wheat-based diet. Broilers were fed the following diets at either 7.9, or 9 g/kg of dietary Ca with: 1) a negative control wheat-based diet, 3.15 g/kg available phosphorus (P) (N...

A total of 720 straight-run Heritage 56 M × fast feathering Cobb 500F broiler chickens was fed from 11 to 13 d of age to determine the impacts of dietary calcium (Ca), phytate phosphorus (PP), and phytase concentrations on inositol phosphate (IP3-6) profile in different digestive tract (GI) segments. The experiment was a 2 × 2 × 3 randomized block design with 2 Ca (0.7 and 1.0%) and 2 PP (0.23 ...