Gentiana pneumonanthe L. (fam. Gentianaceae) is a herbaceous perennial growing on wet heathlands, grasslands and hay meadows of Europe and Asia. Like other Gentiana species, G. pneumonanthe is characterized by the production of bitter secoiridoid glycosides (SGs) widely used in the pharmaceutical industry. Hairy roots (HRs) of G. pneumonanthe, as an alternative source of these phytochemicals, h...

two experiments were performed for in vitro establishment, proliferation and shoot growth in axillary bud explants of sebri pear cultivar. in the first experiment, the effect of different concentrations of iba(indole-3-butyric acid) on explants’ establishment was evaluated. in a second experiment, the influence of several combinations of pgrs (plant growth regulators)on shoot proliferation and ...

Plants growth regulators, especially auxins and cytokinins are critical for in-vitro plant regeneration. The effect of plant growth regulators on the in-vitro propagation of Senna alata (Linn) was investigated. Mature seeds were collected from the medicinal garden of National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan (Lat.7022I N and long.3050I E). Seeds of the species we...

AbstrakSaat ini, perbanyakan rami menggunakan rizoma sebagai bahan tanamnya, tetapi dalam produksinya membutuhkan waktu yang lama dan tanam umur simpannya singkat. Kultur jaringan merupakan salah satu teknologi untuk mendapatkan seragam sehat Tujuan dilakukannya penelitian ini adalah mengetahui pengaruh konsentrasi BAP terhadap pertumbuhan tunas aksilar pada klon lokal Wonosobo mana memberikan ...

Ironwort / Mountain Tea (Sideritis raeseri Boiss & Heldr.) is an endangered (EN) plant species in Albania. This study aimed to develop a rapid clonal propagation protocol using vitro methodologies. The ironwort seeds were pre-treated with three concentrations of GA3 (250, 500, and 1000 mg l-1). During the inoculation stage, two types culture media, Murashige Skoog (MS) Woody Plant Medium (W...

The aim of the present investigation was to develop an efficient and reproducible in vitro regeneration protocol for Brassica campestris L. var. Tori-7. is one most popular major oil yielding crop plants around world including Bangladesh. For this plant, MS medium supplemented with various concentrations 6-benzylaminopurine (BAP) naphthalene acetic acid (NAA) were used. highest frequency callus...

Sugarcane (Saccharum officinarum L.) is the most important food and energy crop worldwide. In present study, an efficient Agrobacterium mediated transformation regeneration system for sugarcane cultivar SPF-234 was established. tumefaciens strains EHA101and LBA4404 using vector pIG121 Hm, having GUS, HPTII NPTII genes were used. Polymerase chain reaction (PCR) histochemical assays confirmed GUS...

Fritillaria meleagris is a horticulturally and medicinally valuable bulbous plant that requires period of low temperatures for proper growth flowering. Since conventional methods propagation are ineffective very slow, tissue culture techniques offer an integrated approach to mass production this geophyte. In study, we investigated the effects various auxin–cytokinin combinations on different mo...

tissue culture methods provide tools to supplement traditional methods for collection, propagation and preservation of endangered plant species. in this study, in vitro propagation of diaphanoptera khorasanica rech.f., a rare and threatened plant species with limited distribution range and population was investigated. this species has a potential as an ornamental plant. single node explants wer...