To digitally analyze expression levels of multiple genes in one reaction, we proposed a method termed as 'MDHB' (Multiplexed Digital-PCR coupled with Hydrogel Bead-array). The template for bead-based emulsion PCR (emPCR) was prepared by reverse transcription using sequence-tagged primers. The beads recovered from emPCR were immobilized with hydrogel to form a single-bead layer on a chip, and th...

suspension polymerization processes are significantly important in polymer bead formation. one of the important factors for bead size control in these processes is variation in surface tension. in this research, the prediction of optimum concentration of a surface active agent was investigated on the basis of the experimental surface tension diagrams. the experimental data show that the desired...

Conventional electron microscopy and rotary shadowing techniques have provided conflicting interpretations of microfibril ultrastructure. To address this issue, we have used quick-freeze deep-etch (QFDE) microscopy to obtain 3-dimensional surface views of microfibrils that have not been fixed, dehydrated, or stained with heavy metals. By this approach, microfibrils appear as tightly packed rows...

We describe a bead-based, multiplexed, oligonucleotide ligation assay (OLA) performed on the Luminex flow cytometer. Differences between this method and those previously reported include the use of far fewer beads and the use of a universal oligonucleotide for signal detection. These innovations serve to significantly reduce the cost of the assay, while maintaining robustness and accuracy. Comp...

The adoption of cytometric bead arrays (CBA) to simultaneously assess the concentrations of multiple cytokines in a single sample has been a significant technological advance in the field of cytokine biology. In this report we extend the use of CBA technology by using the ImageStream system to image cells in flow while at the same time measuring cytokine concentrations. Cytokine levels and cell...

AIM To present validation studies performed for the implementation of existing and new technologies to increase the efficiency in the forensic DNA Section of the Palm Beach County Sheriff's Office (PBSO) Crime Laboratory. METHODS Using federally funded grants, internal support, and an external Process Mapping Team, the PBSO collaborated with forensic vendors, universities, and other forensic ...

The analysis of intracellular proteins from bacterial cells typically requires a cell population of ~1000 or more due to the small cell size. Effective combination of concentration and lysis steps is desired for such analysis to be carried out on a microfluidic platform. In here, we report a simple microfluidic device that integrates the capture of the bacterial cells using a microscale bead ar...