Antibody conjugates are extremely useful reagents for probing many biologically and chemically important molecules in vitro or in vivo. This unit presents some basic protocols for conjugating antibodies with fluorescent dyes, with biotin (see Basic Protocol 1), and with enzymes (see Basic Protocol 2). These protocols have been selected because they are relatively easy to perform and have high r...

Dyes are the major toxic substances in all industrial wastewater and are highly carcinogenic to human beings and other soil and water living things. In this study, the most efficient bacterial isolate in decolorization of Basic Red 46 dye, isolate A3, was isolated and identified as Enterobacter cloacae sp. under the accession number KP202719. Different parameters such as dye concentration (100 ...

The present work describes the development of a voltammetric sensor for the selective determination of Acid Green 25 (AG25) hair dye, widely used in commercial temporary hair dyes. The method is based on a glassy carbon electrode modified with multiwalled carbon nanotubes activated in the presence of sulfuric acid, where the anthraquinone group present as a chromophore in the dye molecule is re...

Cationic dye - basic red 2 (BR2) was removed from aqueous solutions by sulfuric acid activated waste Gossypium hirsutum seeds (WGSAB). The main and interactive effects of five process variables such as, adsorbent dose (1.25 – 5 g/L), initial dye concentration (100–300 mg/L), contact time (1–3 h),  pH (2 – 12) and temperature (20 – 400C) were investigated via  response su...

Palygorskite clay has been investigated as low cost and ecofriendly adsorbent for the removal Basic Red 2(BR-2) from aqueous solution. Batch adsorption studies are carried out by observing effect of amount of adsorbent dose, contact time, pH, initial concentration of ( BR-2) and particle size of adsorbent on the adsorption capacity of the adsorbent were studied. Adsorption data fits the Langmui...

To obtain basic information on the detection of cellulolytic activity in Auricularia auricula-judae, the influences of dye reagent, pH, and temperature were assessed. Chromogenic dye (congo red, phenol red, remazol brilliant blue, and trypan blue) was individually incorporated into a medium containing either carboxymethyl-cellulose, Avicel, or D-cellobiose as a polysaccharide carbon substrate. ...

Due to its unique properties, iron hematoxylin has been traditionally recommended for staining intestinal protozoa. This process can be simplified by reducing the number of steps and periods of permanence of the slides in some of the liquids used, without detriment to the quality of the results. Thus iron hematoxylin becomes adequate for routine use. Hematoxylin is a natural dye extracted from ...

We have developed a simplified version of the side-pumped pulsed dye laser which has a spectral halfwidth of 1.25 GHz and a peak power of 10 kW at 600 nm. The basic laser consists of only four components (output mirror, dye cell, diffraction grating, and tuning mirror) and is exceptionally easy to align. Since the beam expander has been eliminated, the laser cavity can be made quite compact. Un...

In this work, we demonstrate the use of a single DNA strand and G-quadruplex-specific dye NMM as a label-free switch for the construction of series of basic logic gates (YES, NOT, OR, INHIBIT, NOR, AND). The simple GT-rich sequence could be used to interact with several molecules (K(+), thrombin, Hg(2+), and Pb(2+)) to form different structures that can be distinguished by the label-free dye NM...

Electrocoagulation (EC) in a batch cell with Al anode and Fe cathode in monopolar parallel (MP) connection was used for the removal of basic dye, Bromophenol Blue (BPB). The effects of current density, pH, temperature and initial dye concentration, on the process were investigated. Equlibrium data were analyzed using four model equations: Langmuir, Freudlinch, Temkin and Dubinin–Radushkevich. D...