Aim. Callus is a risk factor, leading to severe diabetic foot ulcer; thus, prevention of callus formation is important. However, normal stress (pressure) and shear stress associated with callus have not been clarified. Additionally, as new valuables, a shear stress-normal stress (pressure) ratio (SPR) was examined. The purpose was to clarify the external force associated with callus formation i...

Partially expanded male catkins of swamp white oak (Quercus bicolor Willd.) and red oak (Quercus rubra L.) were cultured on Murashige and Skoog (MS) medium supplemented with BA or 2,4-D. Explants on 2,4-D produced a yellow embryogenic callus originating from the junction of the pedicel and peduncle. Subsequent transfers to MS with BA and then MS without growth regulators resulted in callus prol...

In plants, multiple detached tissues are capable of forming a pluripotent cell mass, termed callus, when cultured on media containing appropriate plant hormones. Recent studies demonstrated that callus resembles the root-tip meristem, even if it is derived from aerial organs. This finding improves our understanding of the regeneration process of plant cells; however, the molecular mechanism tha...

Protein patterns of callus from corn (Zea mays L.) inbreds that are either resistant or susceptible to fall armyworm (Spodoptera frugiperda [J.E. Smith]) were analyzed by two-dimensional electrophoresis. Fall armyworm larvae reared on callus initiated from resistant inbreds were significantly smaller than those reared on callus of susceptible inbreds. A 33-kD protein found in callus from the re...

Studies were conducted on the effect of amino acids and growth regulators on the production of artemisinin, an antimalarial compound, in the callus of Artemisia absinthium. Callus was initiated on solid MS medium supplemented with BAP and NAA from leaf explant. For the production of artemisinin, the callus was proliferated on sterile filter paper bridge in MS medium supplemented with different ...

Through observations of smear materials under a dissecting microscope, it was found that small smooth protuberances were produced in regions showing the dry appearance. Trichomes occurred in the regions showing the green color or the white and green color. There were tracheary elements having thickened wall in the inner part of callus, adjacent to which chloroplasts were observed11>. Scanning e...

The induction of callus formation in cultured buds of Shamouti orange (Citrus sinensis [L.] Osbeck) by abscisic acid (ABA) is a multiphasic process. (Altman, and Goren 1974 Physiol Plant 32: 55.) A study of the mediation by ethylene on this effect of ABA was undertaken. It was found that: (a) ethylene and (2-chloroethyl) phosphonic acid, as well as ABA, induced callus formation; (b) callus indu...

The development of software applications that assist the radiographic evaluation of fracture healing could advance clinical diagnosis and expedite the identification of effective treatment strategies. A radiographic feature regularly used as an outcome measure for basic and clinical fracture healing research is new bone growth, or fracture callus. In this study, we developed OrthoRead, a portab...

Ultrasound is useful in demonstrating bone mineral density of regenerating osseous tissue as well as structural alterations. A proposed ultrasound method, which included ultrasonography and acoustic parameters measurement, was employed to evaluate its efficacy in monitoring the bone callus changes in a rabbit tibial distraction osteogenesis (DO) model. The findings demonstrated that ultrasonogr...