INTRODUCTION It has been demonstrated that organic content of the root canals can influence the antimicrobial capability of chemical irrigants. The aim of this study was to evaluate the effect of bovine serum albumin (BSA), as an organic material, on the antimicrobial activity of several intracanal irrigants. MATERIALS AND METHODS Bactericidal activity of Ethylenediaminetetraacetic acid (EDTA...

Bactericidal antibody is required for direct complement-mediated killing of most pathogenic gram-negative bacteria. Although it has been implicitly assumed that bactericidal antibody functions by increasing complement activation and deposition on the bacterial surface, this assumption may not be valid. We have shown previously (1) that bactericidal IgG increases the bactericidal efficiency of C...

Bactericidal antibody is required for direct complement-mediated killing of most pathogenic gram-negative bacteria. Although it has been implicitly assumed that bactericidal antibody functions by increasing complement activation and deposition on the bacterial surface, this assumption may not be valid. We have shown previously (1) that bactericidal IgG increases the bactericidal efficiency of C...

The rate of inactivation of metronidazole in vitro was determined during the course of time-kill curves against anaerobic and aerobic bacteria in the stationary phase of growth. Metronidazole at a concentration of 10 mug/ml, as measured by bioassay, was rapidly inactivated in broth culture by susceptible anaerobic bacteria (minimum bactericidal concentration </= 3 mug/ml), and this correlated c...

Clinical isolates of Providencia and Proteus with relative aminoglycoside resistance were tested for susceptibility to combinations of gentamicin or tobramycin with cephalothin or cefazolin. The minimal bactericidal concentration of aminoglycoside for one-third of the strains was reduced by fourfold or more in the presence of one-fourth of the minimal bactericidal concentration of either cephal...

Bactericidal activities of HMR 3647 (HMR), moxifloxacin (MXFX), and rifapentine (RPT) against Mycobacterium leprae, measured by the proportional bactericidal technique in the mouse footpad system, were compared with those of the established antileprosy drugs clarithromycin (CLARI), ofloxacin (OFLO), and rifampin (RMP). Administered in five daily doses of 100 mg/kg of body weight, HMR appeared s...