Bacteria were released and separated from soil by a simple blending-centrifugation procedure. The percent yield of bacterial cells (microscopic counts) in the supernatants varied over a wide range depending on the soil type. The superantants contained large amounts of noncellular organic material and clay particles. Further purification of the bacterial cells was obtained by centrifugation in d...

cholera toxin b subunit (ctb) has been extensively studied as an immunogen, adjuvant, and inducer of oral tolerance in many investigations. production of ctb has been carried out in the bacterial, plant, insect and yeast expression systems. in this study the expression of the ctb containing a 6xhis-tagged was performed by escherichia coli (e.coli) m15. the yield of purified pentameric recombina...

BACKGROUND D-phenylglycine aminotransferase (D-PhgAT) of Pseudomonas stutzeri ST-201 catalyzes the reversible stereo-inverting transamination potentially useful in the application for synthesis of D-phenylglycine and D-4-hydroxyphenylglycine using L-glutamate as a low cost amino donor substrate in one single step. The enzyme is a relatively hydrophobic homodimeric intracellular protein difficul...

BACKGROUND Efficient expression systems exist for antibody (Ab) molecules, which allow for characterization of large numbers of individual Ab variants. In contrast, such expression systems have been lacking for soluble T cell receptors (TCRs). Attempts to generate bacterial systems have generally resulted in low yields and material which is prone to aggregation and proteolysis. Here we present ...

Inoculation of wheat seeds with two strains of Pseudomonas fluorescens (III107 and II21) and two strains of Bacillus mycoides (JC192 and K184) isolated from winter wheat roots, as well as with one strain of P fluorescens (ID13) isolated from oat roots, reduced the negative influence of Fusarium culmorum on winter wheat in a 28 day pot experiment. The bacterial strains (especially III107 and chi...

A bioenergetic model has been developed to examine growth kinetics associated with bacterial utilization of dissolved organic matter (DOM), NH4+, and N03-. A set of 11 metabolic reactions are used to govern the incorporation, oxidation, and N remineralization of DOM and dissolved inorganic N associated with bacterial growth. For each reaction, free energies and electron transfer requirements ar...

Agaricus bisporus has a high nutritional value and health benefits its popularity is increasing among vegans health-conscious consumers, indicating the need for stable production. Therefore, we examined bacterial flora of substrates used to produce A. using 16S rRNA gene ana­lysis discussed relationship between yield. The results obtained showed that yield slightly decreased with an increase in...