نتایج جستجو برای: bacillus thuringiensis var kurstaki

تعداد نتایج: 81473  

Journal: :Journal of bacteriology 1987
A Heierson R Landén A Lövgren G Dalhammar H G Boman

Plasmid DNA-mediated transformation of vegetative cells of Bacillus thuringiensis was studied with the following two plasmids: pBC16 coding for tetracycline resistance and pC194 expressing chloramphenicol resistance. A key step was the induction of competence by treatment of the bacteria with 50 mM Tris hydrochloride buffer (pH 8.9) containing 30% sucrose. Transformation frequency was strongly ...

Journal: :Applied and environmental microbiology 1991
T M Walter A I Aronson

A derivative of Bacillus thuringiensis subsp. kurstaki HD1 (HD1-9) released transducing phage (TP21) from late exponential cultures. Three of seven markers tested were transduced into Bacillus cereus, but only two of these (cysC and trpB/F) were transduced at a frequency of more than 100 times the reversion rates. A limited transduction capacity was given further support in that few chromosomal...

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