Many bacteria concentrate their chemoreceptors at the cell poles. Chemoreceptor location is important in Escherichia coli, since chemosensory responses are sensitive to receptor proximity. It is not known, however, whether chemotaxis in other bacteria is similarly regulated. To investigate the importance of receptor-receptor interactions in other bacterial species, we synthesized saccharide-bea...

The aim of the present investigation was to assess and compare the antimicrobial activities of the lemon (Citrus sinensis) peel extract and lemon oil against common food borne pathogens and to study their potential as food bio-preservatives. The antagonistic activity of lemon peel extract and lemon oil was studied against common food borne pathogens like Staphylococcus aureus, Staphylococcus ep...

Single-stranded DNA-binding protein (SSB) is a well characterized ubiquitous and essential bacterial protein involved in almost all aspects of DNA metabolism. Using the Bacillus subtilis SSB we have generated a reagentless SSB biosensor that can be used as a helicase probe in B. subtilis and closely related gram positive bacteria. We have demonstrated the utility of the probe in a DNA unwinding...

We report a previously undescribed quorum-sensing mechanism for triggering multicellularity in Bacillus subtilis. B. subtilis forms communities of cells known as biofilms in response to an unknown signal. We discovered that biofilm formation is stimulated by a variety of small molecules produced by bacteria--including the B. subtilis nonribosomal peptide surfactin--that share the ability to ind...

The min system prevents polar cell division in bacteria. Here, the biochemical characterization of the interaction of MinC and FtsZ from a Gram-positive bacterium, Bacillus subtilis, is reported. B. subtilis MinC inhibits FtsZ polymerization in a pH-dependent manner by preventing the formation of lateral associations between filaments. The inhibitory effect of MinC on FtsZ polymerization is cou...

The antimicrobial efficacy of methanol, ethyl acetate, chloroform and butanol extracts of fungus Chaetomium cupreum was determined by employing well diffusion and broth micro-dilution techniques. Chaetomium species are the largest genus of saprophytic ascomycetous fungus, which belongs to the class Sordariomyetes and family Chaetomiaceae. Ethyl acetate and butanol extracts exhibited antibacteri...

In this study, 5,10,15,20-tetrakis(4-nitrophenyl)porphyrin (TNPP) and its zinc porphyrin complex (ZnTNPP) were synthesized in situ using the microwave method and identified by UV-Vis, FT-IR and 1H NMR. The photostability and photodynamic antimicrobial activity (PACT) of these compounds were investigated on Pseudomonas aeruginosa and Bacillus subtilis under visible light irradiation. MIC, MBC an...

Herein, CoF2 nanoparticles (NPs) are prepared by simple coprecipitation method and characterized various techniques, i.e., XRD, SEM/EDX, FTIR, UV/Vis, for their structure identification. As-prepared nanostructures were used as photocatalyst, antioxidant, antimicrobial agent. The degradation studies of the samples carried out specific time methylene blue (MLB) dye under a UV/visible spectrophoto...

Biofilm is formed by one or different types of microbes that can grow on surfaces. Bacteria, fungi, and many protists form biofilms. formation requires cell to signaling mechanism which defined as quorum sensing. In this work, we have studied about the antibiofilm activity methanolic extract nutmeg against Gram-positive Bacillus subtilis bacteria. Our test inhibited biofilm production well erad...

Rabbits receiving repeated intravenous injections of killed bacteria (Escherichia coli or Bacillus subtilis) developed IgM rheumatoid factor which reacted with autologous heat-aggregated IgG. In addition, 5/7 'Old English' and 7/8 'Sandy Lop' rabbits receiving killed E. coli developed rheumatoid-like synovial lesions. 'Old English' rabbits developed lesions of a more severe nature. Three of eig...