The members of the Gram-positive endospore-forming bacteria that made up the genus Bacillus have been gradually subdivided, during the last few years, into a number of new genera such as Alicyclobacillus, Aneuribacillus, Brevibacillus, Gracilibacillus, Paenibacillus, Salibacillus, Ureibacillus, and Virgibacillus. Nazina et al. in 2001 created the genus Geobacillus based around Bacillus (now Geo...

The soil-related Bacillus and Paenibacillus species have increasingly been implicated in various human diseases. Nevertheless, their identification still poses problems in the clinical microbiology laboratory and, with the exception of Bacillus anthracis and Bacillus cereus, little is known on their pathogenicity for humans. In this study, we evaluated the use of matrix-assisted laser desorptio...

BACKGROUND Biosurfactants have been reported to utilize a number of immiscible substrates and thereby facilitate the biodegradation of panoply of polyaromatic hydrocarbons. Olive oil is one such carbon source which has been explored by many researchers. However, studying the concomitant production of biosurfactant and esterase enzyme in the presence of olive oil in the Bacillus species and its ...

The methods suggested for the identification of species of the genus Bacillus in the invaluable monographs of Smith et al. (1946, 1952) have been applied to a collection of named cultures, and also have been utilized for the classification of approximately 400 strains of these organisms isolated from samples of blood, spinal fluid, surgical tissue, and other human and animal sources, as well as...

The complete sequencing and annotation of the 181.7-kb Bacillus anthracis virulence plasmid pXO1 predicted 143 genes but could only assign putative functions to 45. Hybridization assays, PCR amplification, and DNA sequencing were used to determine whether pXO1 open reading frame (ORF) sequences were present in other bacilli and more distantly related bacterial genera. Eighteen Bacillus species ...

Spore-bearing aerobic bacteria' capable of producing striking black pigments have been known since the early reports of Gorini (1896) on "Bacillus lactis-niger" and of Biel (1896) and Lunt (1896) on "Bacillus mesentericus-niger." Although these types have become established in the bacteriological literature under the respective binomial designations of Bacillus niger (Migula, 1900) and Bacillus...

Eight Bacillus strains isolated from Sonoran Desert soil were shown to belong to a previously unidentified species, for which the name Bacillus sonorensis sp. nov. is proposed. The type strain is strain L87-10T (= NRRL B-23154T). On the basis of phenotypic and genetic data, B. sonorensis is most closely related to Bacillus licheniformis. B. sonorensis can be distinguished from B. licheniformis ...

Five strains of Gram-positive, endospore-forming, moderately halotolerant bacteria were studied taxonomically. Four were isolated from Vietnamese fish sauce and one from the Gulf of Mexico. Phylogenetic analysis based on 16S rRNA gene sequences showed that these strains clustered within the radiation of the genus Bacillus but separately from recognized Bacillus species. DNA G+C composition of t...

The diversity of Bacillus species isolated from the fermented soup condiment okpehe in Nigeria was studied using a combination of phenotypic and genotypic methods. Fifty strains presumptively characterized as Bacillus spp. using the API 50 CHB test were further identified by PCR of randomly amplified polymorphic DNA (RAPD) and by amplified ribosomal DNA restriction analysis (ARDRA) genotyping m...