The role of bovine mammary macrophages and peripheral blood monocyte-derived macrophages in natural resistance to bovine brucellosis was evaluated. A group of 11 naturally resistant and 10 chronically infected susceptible cows was studied following challenge with Brucella abortus. Macrophages from a greater proportion (P less than 0.026) of naturally resistant cows were significantly superior t...

Clinical diagnosis of canine brucellosis is not sensitive enough and a negative blood culture cannot rule out the disease. Indirect methods of serological testing such as agar gel immunodiffusion (AGID), rapid slide agglutination test (RSAT) and indirect enzyme linked immunoassay (IELISA) are preferred for routine diagnosis. Since Brucella canis shares antigenic components with the Brucella ovi...

Brucellosis is an infectious zoonotic disease of domestic and wild animals. It has a serious implication on human health as well as on the economic development in a developing country like India. Rapid identification and treatment of the disease in human and animal is important for the prevention of spreading the disease. Isolation with a conventional bacteriological method for the identificati...

Brucella abortus is an intracellular pathogen that persists within phagocytic cells of the reticuloendothelial system. To identify in vivo interactions between B. abortus and the host that lead to persistent infection, we studied the persistence of B. abortus and an isogenic virB mutant deficient in the VirB type IV secretion system (T4SS) in knockout mice. In contrast to control mice, mice lac...

The requirement of many Brucella abortus strains for a PCO2 of approximately 0.03 atmosphere for growth seems to differ both quantitatively and qualitatively from the C02 requirement of other heterotrophic organisms. In some microorganisms, C02 can be replaced by certain tricarboxylic acids (Lwoff and Monod, 1946; Ajl and Werkman, 1948), amino acids (Lyman et al., 1947; Abelson et al., 1952a, b...

Brucella abortus is an intracellular pathogen of monocytes, macrophages, dendritic cells, and placental trophoblasts. This bacterium causes a chronic disease in bovines and in humans. In these hosts, the bacterium also invades neutrophils; however, it fails to replicate and just resists the killing action of these leukocytes without inducing significant activation or neutrophilia. Moreover, B. ...

The strategies that allow Brucella abortus to survive inside macrophages for prolonged periods and to avoid the immunological surveillance of major histocompatibility complex class II (MHC-II)-restricted gamma interferon (IFN-gamma)-producing CD4+ T lymphocytes are poorly understood. We report here that infection of THP-1 cells with B. abortus inhibited expression of MHC-II molecules and antige...

We have previously identified a Brucella melitensis 28 kDa cytosoluble protein (CP28) which was highly immunogenic in infected sheep and which in addition made possible the serological differentiation between infected and B. melitensis Rev. 1 vaccinated sheep. Monoclonal antibodies against CP28 were used to screen a B. melitensis 16M genomic library and to clone the corresponding gene. DNA sequ...

background: brucella abortus is a gram-negative facultative intracellular bacterium that can cause a highly contagious disease in sheep, goats, cattle and one-humped camels. it is responsible for one of the most important zoonosis in human. the aim of this study was to evaluate the role of mentha piperita, origanum majorana, citrus lemon, cinnamomum verum and myristica fragrans essential volati...

To determine the role of different Brucella (B.) spp. in Bangladesh, 62 animal samples and 500 human sera were tested. Animal samples from cattle, goats and sheep (including milk, bull semen, vaginal swabs and placentas) were cultured for Brucella spp. Three test-positive human sera and all animal samples were screened by Brucella genus-specific real-time PCR (RT-PCR), and positive samples were...