Two experiments were conducted to evaluate the effects of microwaving, autoclaving (moist heating), and roasting (dry heating) on gas production parameters and in situ rumen degradability of soybean meal (SBM). SBM was treated by roasting at 140℃ for 30 and 60 min, and at 160℃ for 30 and 60 min, autoclaving at 121℃ for 20 min, and microwaving for 2, 4, and 6 min. A gas production trial...

Ribonucleases (RNases) are ubiquitous enzymes that can detrimentally affect RNA isolation in the laboratory. Many applications such as RT-PCR and in vitro transcription and translation may be affected as well. RNase can renature after autoclaving and is difficult to inactivate. Many RNase inhibitors are available commercially. Promega’s RNasin® Ribonuclease Inhibitor, one of the first introduce...

sterilized soils are commonly used for the study of xenobiotic sorption and as an abiotic control in biodegradation experiments. they are also used for the chemical study of nitrogen and carbon cycle processes and the elimination of the biological factors. in this research the effects of different soil sterilization methods such as autoclaving, fumigating and exposure to ultraviolet and microwa...

Numerous recipes exist for the clearing of plant tissues prior to examination as whole mounts (see annotated bibliography by Lersten 1967). Broadly speaking, these methods may be classified into 3 groups; mild, drastic and very drastic procedures, depending upon the severity with which the tissue is extracted by the clearing procedure. For some purposes mild procedures are all that are needed f...