The K(+)-stimulated ATPase was partially purified from a plasma membrane fraction of suspension cultured cells of rose (Rosa damascena) by two different solubilization procedures. Solubilization with 30 mm octyl-beta-d-glucopyranoside followed by precipitation with ammonium sulfate increased the specific activity of the enzyme about 6-fold. Solubilization with 1% cholate removed all but 1% of t...

1 Effects of various frequencies (0.25, 0.5, 1.0, 1.5 or 2.0 Hz) of stimulation for various durations (2, 5, 10 or 15 min) on the contractile force of trabecular or papillary muscles of dog myocardium were investigated. 2 Effects of various frequencies (0, 0.25, 0.5, 1.0, 2.0 Hz) of various stimulus strengths (0.5, 1, 10 V) for various durations (2,5,10 or 15 min) on the Mg2+/-dependent Na+/--K...

In the present study we have localized acid and alkaline phosphatases, glucose-6-phosphatase and sodium-potassium ATPase (Na-K-ATPase) in ultra-thin sections of cerebral neurosecretory cells and oesophageal cells of the earthworm, Aporrectodea caliginosa. The majority of the enzymes studied were located in the rough endoplasmic reticulum (RER), nuclear envelope and Golgi apparatus.

The Mg2+ + Ca2+ activated ATPase of sarcoplasmic reticulum can be visualized by negative staining with uranyl acetate or Kc-phosphotungstate in the form of 40 a diameter surface particles (I), and by freeze fracture as 85 A diameter intramembranous particles (2), which are more numerous in the cytoplasmic than in the luminal fracture face. In sarcoplasmic reticulum and in reconstituted ATPase v...

this study was carried out to determine the appropriate size of caspian trout ( salmo caspius kessler, 1877) juveniles for releasing into the rivers that enter south of the caspian sea for stock enhancement, or possibility of cage culture in the caspian sea. a total of 1611 specimens in 4 weight groups of 5, 10, 15 and 20g were exposed to 3 salinity trials which include caspian sea water (11- 1...

The Na,K-ATPase is essential for the contractile function of skeletal muscle, which expresses the α1 and α2 subunit isoforms of Na,K-ATPase. The α2 isozyme is predominant in adult skeletal muscles and makes a greater contribution in working compared with noncontracting muscles. Hindlimb suspension (HS) is a widely used model of muscle disuse that leads to progressive atrophy of postural skeleta...

objective: the urinary system of euryhaline fish, as well as the gills, is involved in ion regulation through the production of dilute urine in freshwater and isotonic urine in seawater. the low osmolality of the urine originates from an active reabsorption of ions by ionocytes or mitochondria rich-cells (mrcs) present in certain parts of the urinary system. mitochondria rich-cells possess a hi...

PRR [(pro)renin receptor] was named after its biological characteristics, namely the binding of renin and of its inactive precursor prorenin, that triggers intracellular signalling involving ERK (extracellular-signal-regulated kinase) 1/2. However the gene encoding for PRR is named ATP6ap2 (ATPase 6 accessory protein 2) because PRR was initially found as a truncated form co-purifying with V-ATP...