Background: The lipids of the spermatozoa membrane are important for the fluidity and flexibility of spermatozoa. However, spermatozoa’s lipids are the main substrates for peroxidation, which may provoke severe functional disorder of sperm. Objective: The aim of this study was to investigate the fatty acids composition of spermatozoa in men with asthenozoospermia, asthenoteratozoospermia and ol...

HOTAIR, a long noncoding RNA, regulates development and progression of tumor cells and function of normal stem cells. However, the role and the molecular mechanism of HOTAIR in the spermatozoa of patients with asthenozoospermia and oligoasthenozoospermia are still unclear. Herein, 45 healthy control, 45 asthenozoospermic patients and 45 oligoasthenozoospermic patients were enrolled. Initially, ...

Mitochondria of spermatozoa are different from the corresponding organelles of somatic cells, in both their morphology and biochemistry. The biochemical differences are essentially related to the existence of specific enzyme isoforms, which are characterized by peculiar kinetic and regulatory properties. As mitochondrial energy metabolism is a key factor supporting several sperm functions, thes...

Copyright @ 2015 Authors. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercialShareAlike 4.0 International License (http://creativecommons.org/licenses/by-nc-sa/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are properly cited. Silvia W. Lestari,1,...

BACKGROUND Asthenozoospermia is one of the most common findings present in infertile males characterized by reduced or absent sperm motility, but its aetiology remains unknown in most cases. In addition, calcium is one of the most important ions regulating sperm motility. In this study we have investigated the progesterone-evoked intracellular calcium signal in ejaculated spermatozoa from men w...

The mammalian testis is a complex organ which produces spermatozoa and synthesizes steroids. The transformation of androgens into estrogens is catalyzed by aromatase, an enzymatic complex encoded by a single copy-gene (cyp19) which contains 18 exons, 9 of them being translated. In man besides Leydig cells, we have demonstrated the existence of a biologically active aromatase in immature germ ce...

INTRODUCTION Optical trapping is a laser-based method for probing the physiological and mechanical properties of cells in a noninvasive manner. As sperm motility is an important criterion for assessing the male fertility potential, this technique is used to study sperm cell motility behavior and rotational dynamics. METHODS AND PATIENTS An integrated optical system with near-infrared laser be...

OBJECTIVE To explore potential relationships between sperm DNA integrity and both semen parameters and clinical outcomes. METHODS Semen analysis of 498 samples was performed according to the 2010 criteria of the World Health Organization. The sperm DNA fragmentation Index (DFI) of the semen samples was assessed using a neutral comet assay. RESULTS Sperm DFI showed a significant correlation ...

NADþ-dependent Isocitrate Dehydrogenase (NADþ-IDH) could be one of the cell phone radiation targets. Enzyme activity alteration may lead to decline in sperm motility during radio-frequency electromagnetic waves (RF-EMW) exposure. The current case control study aimed to investigate the possible relationship between mitochondrial NADþ-IDH activity in human seminal plasma and sperm motility among ...