Results from application of reversed-phase HPLC with electrochemical detection to analysis of catecholamines and total antioxidant potential are presented. The assay was used to estimate their daily profiles in blood serum of patients treated with anti-Parkinson drugs. It was found that increases in the concentration of hydroxy radicals in blood serum, and of the total antioxidant potential rel...

The stability-indicating LC assay method was developed and validated for quantitative determination of cefcapene pivoxil in the presence of degradation products formed during forced degradation studies. An isocratic RP-HPLC method was developed with a Lichrospher RP-18 (250 mm × 4.6 mm, 5 μm) column and the mobile phase composed of 45 volumes of acetonitrile and 55 volumes of mixture composed o...

Some studies have suggested that the immunogenicity of oats depends on the cultivar. RP-HPLC has been proposed as a useful technique to select varieties of oats with reduced immunogenicity. The aim of this study was to identify both the avenin protein patterns associated with low gluten content and the available variability for the development of new non-toxic oat cultivars. The peaks of alcoho...

A simple stability-indicating RP-HPLC/UV method was validated for determination of beclomethasone dipropionate (BD) in nanocapsule suspensions. Chromatographic conditions consisted of a RP C18column (250 mm x 4.60 mm, 5 μm, 110 Å), using methanol and water (85:15 v/v) as mobile phase at 1.0 mL/ min with UV detection at 254 nm. The calibration curve was found to be linear in the concentration ra...

A reliable and sensitive isocratic stability indicating RP-HPLC method has been developed and validated for assay of rosuvastatin calcium in tablets and for determination of content uniformity. An isocratic separation of rosuvastatin calcium was achieved on YMC C8, 150×4.6 mm i.d., 5 μm particle size columns with a flow rate of 1.5 ml/min and using a photodiode array detector to monitor the elu...

A novel stability indicating RP-HPLC method having a very short run time was developed and validated for determination of assay of Voriconazole in bulk solution, lyophilized injectable dosage form and in physiological compatible solutions. The method is novel, rapid, precise, accurate and capable of separating known impurities and degradants from Voriconazole peak proving the stability-indicati...

A new RP-HPLC method was developed for selective and simultaneous determination of of Desogestrel and Ethinyl Estradiol Tablets. The chromatographic separation was achieved on a Zorbax SB C-18, 4.6 x 250mm, and 5μ column. The gradient LC method employs solutions A and B as mobile phase. The solution A milliQ water and acetonitrile in the ratio of 90:10 and degas and solution B contains a mixtur...

Cereal Chern. 65(3):215-222 Zein, the major alcohol-soluble protein of corn endosperm, was extracted with 55% 2-propanol. It was then fractionated by preparative reversed-phase (CIs) high-performance liquid chromatography (RPH PLC) with an acetonitrile-triOuoroacetic acid gradient. From 15 to 17 peaks resulted for native or reduced and alkylated (R-A) zeins. Sodium dodecyl sulfate-polyacrylamid...

Abstract: Aim: A new stability indicating RP-HPLC based methods were developed to identify the possible degradation products of Ivermectin in isolated rat hepatocytes and different pH media using a C18 RP column. Complete validation, including linearity, accuracy, recovery, precision, robustness, stability, peak purity, was performed. Materials Methods: HPLC system UltiMateDionex 3000 DAD with ...