1. The conductivities of aqueous solutions of glycine, d,l-valine, and l-asparagine have been determined, and comparisons have been made with similar data reported in the literature. 2. On the basis of certain theoretical considerations, calculations of the expected conductivities of aqueous solutions of glycine, asparagine, aspartic acid, and glutamic acid have been made and these data have be...

In a study of minimal requirements for certain lactobacilli, Leuconostoc dextranicum 8086 was observed to require either aspartic acid or threonine for growth in a particular medium. Since such an observation does not allow specific conclusions regarding the relationship of these two amino acids, a study of this interrelationship in lactobacilli in which aspartic acid biosynthesis can be contro...

The multiconformational landscape of the proteogenic amino acid aspartic acid, the simplest amino acid with two carboxylic groups, has been explored for the first time in the gas phase. Solid aspartic acid (m.p. > 300 degrees C) was vaporized by laser ablation and expanded in a supersonic jet where rotational spectra of various conformers were obtained by Fourier transform microwave spectroscop...

In a study of minimal requirements for certain lactobacilli, Leuconostoc dextranicum 8086 was observed to require either aspartic acid or threonine for growth in a particular medium. Since such an observation does not allow specific conclusions regarding the relationship of these two amino acids, a study of this interrelationship in lactobacilli in which aspartic acid biosynthesis can be contro...

Cairomycin B in the fermentation broths of Streptomyces sp. strain AS-C-19 accompanied cairomycin A and cairomycin C. The cairomycins are peptides with potent activity against gram-positive bacteria. On acid hydrolysis, cairomycin A yielded valine and aspartic acid, whereas cairomycin C yielded lysine, glycine, valine, leucine, and aspartic acid, as identified by paper and gas chromatography. T...

Sequence analysis of membrane-bound glycerolipid acyltransferases revealed that proteins from the bacterial, plant, and animal kingdoms share a highly conserved domain containing invariant histidine and aspartic acid residues separated by four less conserved residues in an HX4D configuration. We investigated the role of the invariant histidine residue in acyltransferase catalysis by site-direct...

Recent studies have found that certain urinary proteins can efficiently inhibit stone formation. These discoveries are significant for developing effective therapies for stone disease, but the inhibition mechanism of crystallization remains elusive. In the present study, polyaspartic acid (PASP) was employed as a model peptide to investigate the effect of urinary proteins on the crystallization...

Senecio jacobaea (SJ) was incubated in sheep rumen fluid-buffer mixtures to determine if metabolism and(or) detoxication of pyrrolizidine alkaloids (PA) was occurring. The nontoxic reduction metabolite, 7 beta-hydroxy-l-methylene-8 alpha-pyrrolizidine, was not detected when SJ-rumen fluid incubation extracts were subjected to high performance liquid chromatography and mass spectrographic analys...

We report a new method for the catalytic, asymmetric synthesis of beta-substituted aspartic acid derivatives in which the nucleophilic catalyst serves up to four discrete roles in a one-pot procedure: catalytic dehydrohalogenation of acid chlorides to form ketenes; catalytic dehydrohalogenation of alpha-chloroamines to form the corresponding imines; catalyzed [2 + 2]-cycloaddition to produce in...

A polypeptide of Mr 4400 was isolated from soybean (Glycine max) seeds by extraction with 60% ethanol followed by ion-exchange and reverse-phase chromatography. The peptide contains an unusually high amount of aspartic acid residues (approximately 25%, and hence is designated as soybean aspartic acid-rich peptide). Its complete primary structure was determined by conventional methods to be the ...