The pressing issue of the abundance antibiotic resistance genes and resistant bacteria in environment (ARGs ARB, respectively) requires procedures for assessing risk to health. chemo-centric environmental assessment models identify hazard(s) a dose–response manner, obtaining exposure, toxicity, risk, impact policy. However, this approach based on ARGs/ARB evaluation from quantitative viewpoint ...

The Midwestern United States is dominated by agricultural production with high concentrations of swine, leading to application of swine manure onto lands with artificial subsurface drainage. Previous reports have indicated elevated levels of antibiotic resistance genes (ARGs) in surface water and groundwater around confined animal feeding operations which administer antimicrobials. While previo...

Association, or linkage disequilibrium (LD), mapping is an intensely studied approach to gene mapping (genome-wide or in candidate regions) that is widely hoped to be able to efficiently locate genes influencing both complex and Mendelian traits. The logic underlying association mapping implies that the best possible mapping results would be obtained if the genealogical history of the sampled i...

Debate exists about whether agricultural versus medical antibiotic use drives increasing antibiotic resistance (AR) across nature. Both sectors have been inconsistent at antibiotic stewardship, but it is unclear which sector has most influenced acquired AR on broad scales. Using qPCR and soils archived since 1923 at Askov Experimental Station in Denmark, we quantified four broad-spectrum β-lact...

Plasmids operate as independent genetic elements in microorganism communities. Through horizontal gene transfer (HGT), they can provide their host microorganisms with important functions such as antibiotic resistance and heavy metal resistance. In this study, six metagenomic libraries were constructed with plasmid DNA extracted from influent, activated sludge (AS) and digested sludge (DS) of tw...

It becomes increasingly clear that the basis of antibiotic resistance problem among bacterial pathogens is not confined to the borders of clinical microbiology but has broader ecological and evolutionary associations. This Research Topic “Role and prevalence of antibiosis and the related resistance genes in the environment” in Frontiers in Microbiology: Antimicrobials, Resistance, and Chemother...

Most studies of whether and how nanoparticles (NPs) affect antibiotic resistance genes (ARGs) focus on testing single NPs type. In this study, we determined the combined effect Cu- ZnO- in water samples collected from Yangtze River Estuary found differs greatly that produced by individual NPs. The results showed co-exposure resulted an enrichment ARGs, whereas exposure decreased abundance ARGs....

conclusions our findings revealed that the zfn technology could be employed to overcome ampicillin resistance by the targeted disruption of the ampicillin resistance gene, which leads to inactivation of β-lactam synthesis. therefore, zfn technology could be engaged to decrease the antibiotic resistance issue with the construction of a zfn archive against different args. to tackle the resistance...

Landfills constitute the largest treatment and disposal reservoirs of anthropogenic waste on earth they are continuously releasing antibiotic resistance genes (ARGs) to environment for decades via leachates. Little is known about association between ARGs human bacterial pathogens as a function time. Here, we quantified 10 subtypes ARGs, integrons, (HBPs). Except encoding sulfonamides, beta-lact...

We explored the bacterial diversity of untreated sewage influent samples of a wastewater treatment plant in Tucson, AZ and discovered that Arcobacter cryaerophilus, an emerging human pathogen of animal origin, was the most dominant bacterium. The other highly prevalent bacteria were members of the phyla Bacteroidetes and Firmicutes, which are major constituents of human gut microbiome, indicati...