BACKGROUND The indwelling venous catheter such as Dual-Cath or Twin-Cath is widely used in haemodialysis. Although the manufacturer recommends filling the catheter lumen with heparin after the dialysis session to prevent clotting, little is known about the systemic effects of such a procedure. METHODS Twenty haemodialysis patients with Dual-Cath were studied. Dialysis anticoagulation was achi...

Heparin is frequently used in different clinical settings to reduce the coagulating ability of the blood. Because of probable adverse effects owing to heparin therapy and regarding variability of patients' responses to heparin, which make it very unreliable, it seems prudent to monitor meticulously its effects on the human body. There are a lot of laboratory tests to watch its effects on the bo...

This paper describes extending plug-based microfluidics to handling complex biological fluids such as blood, solving the problem of injecting additional reagents into plugs, and applying this system to measuring of clotting time in small volumes of whole blood and plasma. Plugs are droplets transported through microchannels by fluorocarbon fluids. A plug-based microfluidic system was developed ...

BACKGROUND Plasma-free volume replacement in haemorrhage often results in dilutional coagulopathy. Prothrombin time index (PTI) and activated partial thromboplastin time (aPTT) are used for monitoring haemostasis but have not yet been clinically evaluated. Our aim was to investigate the effects of haemodilution on the course of global coagulation tests and clotting factors (CFs). METHODS Bloo...

Thromb Haemost 2008; 100: 9–10 Downey et al. published a paper in 1997 entitled ‘Novel and diagnostically applicable information from optical waveform analysis of blood coagulation in disseminated intravascular coagulation’ (1). Using a new automated coagulation analyzer, the MDA-180, the authors observed that during measurement of the aPTT, some samples became turbid immediately after addition...

Unfractionated heparin (UFH) remains an important therapeutic modality for anticoagulation in children. Its short half-life and easy reversibility with protamine sulfate make it an attractive option in critically ill children. Although initially described as a screening tool for hemophilia, the aPTT is a global hemostatic assay that reflects the integrity of the intrinsic and common pathways of...

COVID-19患者では，凝固異常が合併しやすいことが知られている．私たちは，ACL-TOP 700 hemostasis testing systemにて，APTT凝固波形解析をしたところ，COVID-19患者では，高率に凝固波形パターン異常がみられることを経験した．その後の報告をみると，当該測定系以外の測定系では，このような波形パターン異常は報告されておらず，測定系特異的な現象と考えられる．一方で，凝固塊の形成速度，加速度を示す指標は，調べた範囲のすべての測定系で上昇しており，COVID-19での凝固異常をAPTT凝固波形解析は反映していると考えられる．その機序としては，現在のところ特定できていないが，凝固波形パターン異常を示す検体では，特にフィブリノゲンが高値を示していた．その他，ループスアンチコアグラント，VWF/ADAMTS13，血中脂質の変動などが要因として考えられる．一...