We studied the mutagenicity of the proximate bladder carcinogen, N-hydroxy-4-acetylaminobiphenyl (N-OH-AABP) in embryonic fibroblasts of the Big Blue mouse. Treatment of these cells with increasing concentrations of N-OH-AABP for 24 h resulted in a dose-dependent increase in mutation frequency of the cII transgene up to 12.8-fold over the background. Single base substitutions comprised 86% of t...

Manivel, V. A. 2017. The role of anti-collagen type II antibodies in the pathogenesis and prognosis of rheumatoid arthritis. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1289. 62 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-554-9792-7. Rheumatoid arthritis (RA) which affects 0.5-1% of the world population and is characterised by joint erosion...

Using the 12-m APEX telescope, we have detected redshifted emission from the 157.74 μm [CII] line in the z = 4.4074 quasar BRI 1335-0417. The linewidth and redshift are in good agreement with previous observations of high-J CO line emission. We measure a [CII] line luminosity, L[CII] = (16.4 ± 2.6) × 109 L , making BRI 1335-0417 the most luminous, unlensed [CII] line emitter known at high-redsh...

We present the detection of bright [CII] emission in the z=4.76 submillimetre galaxy LESS J033229.4-275619 using the Atacama Pathfinder EXperiment. This represents the highest redshift [CII] detection in a submm selected, star-formation dominated system. The AGN contributions to the [CII] and far-infrared (FIR) luminosities are small. We find an atomic mass derived from [CII] comparable to the ...

The small heat shock proteins (sHSPs) and alpha-crystallins are highly effective, ATP-independent chaperones that can bind denaturing client proteins to prevent their irreversible aggregation. One model of sHSP function suggests that the oligomeric sHSPs are activated to the client-binding form by dissociation at elevated temperatures to dimers or other sub-oligomeric species. Here we examine t...

To evaluate the thermo-responsive poly(N-isopropylacrylamide) (PNiPAAm) polymer as an adjuvant, we synthesized PNiPAAm through free radical polymerization and characterized it both in vitro and in vivo. The polymer when mixed with collagen type II (CII) induced antigen-specific autoimmunity and arthritis. Mice immunized with PNiPAAm-CII developed significant levels of CII-specific IgG response ...

The objective of this study was to investigate the catalytic activity of basic aminopeptidase (APB) and its association with periarticular edema and circulating tumor necrosis factor (TNF)-alpha and type II collagen (CII) antibodies (AACII) in a rat model of rheumatoid arthritis (RA) induced by CII (CIA). Edema does not occur in part of CII-treated, even when AACII is higher than in control. TN...

A critical event in an immune response is the T cell recognition of peptides bound to major histocompatibility complex (MHC) molecules on the surface of an antigen presenting cell (APC). Although the majority of eukaryotic proteins are glycosylated, it has not yet been shown that T cell recognition of such proteins involves recognition of the bound carbohydrates. Type II collagen (CII), the maj...

BACKGROUND Collagen-induced arthritis (CIA) is the most commonly used model of rheumatoid arthritis (RA). In both CIA and RA there is an increase in the cellular content of the synovium, this being dominated by macrophages. OBJECTIVE To assess the impact of etoposide, a topoisomerase II antagonist known to induce monocyte apoptosis, on the development of CIA. METHODS Mice were primed and bo...

INTRODUCTION Rheumatoid arthritis (RA) patients with autoantibodies against collagen type II (CII) are characterized by acute RA onset with elevated inflammatory measures and early joint erosions as well as increased production of tumor necrosis factor-α (ΤΝF-α) by peripheral blood mononuclear cells (PBMC) stimulated by anti-CII immune complexes (IC) in vitro. Polymorphonuclear granulocytes (PM...