Sera of some patients with systemic lupus erythematosus and related diseases contain a polyclonal antibody population (cross-reactive antinuclear antibodies [X-ANA]) that react specifically with both core mononucleosomes and plasma membranes of viable nucleated cells. Native mononucleosomes and nucleosome cores assembled from long DNA and the inner histones were indistinguishable in terms of in...

Rowell syndrome is usually diagnosed by the presence of erythema multiforme like targetoid lesions in the patients of cutaneous or systemic lupus erythematosus and characterized by specific serological or immunological profile like speckled antinuclear antibody pattern, antibody against saline extract of human tissues (anti-SjT) and positive serum rheumatoid factor.The absence of a universally ...

PROBLEM Systemic lupus erythematosous (SLE) is a chronic disease with a broad spectrum of clinical and immunological manifestations, therefore, this condition may be frequently misdiagnosed. The current study was conducted to determine the prevalence of undiagnosed SLE among a cohort of infertile women. METHOD OF STUDY Screening was performed using sequential (two stage) testing, which includ...

Indirect immunofluorescence (IIF) on human epithelial (HEp-2) cells is considered as the gold standard screening method for the detection of antinuclear autoantibodies (ANA). However, in terms of automation and standardization, it has not been able to keep pace with most other analytical techniques used in diagnostic laboratories. Although there are already some automation solutions for IIF inc...

The goal of this prospective longitudinal study was to determine the serological profile of early rheumatoid arthritis (RA), and to test whether antikeratin antibody (AKA), antiperinuclear factor (APF), anti-RA33 antibody and antinuclear antibodies (ANA) had an additional diagnostic value when prescribed after rheumatoid factor (RF)-detecting methods. Sixty-nine patients with early polyarthriti...

Systemic sclerosis is a generalized disorder of connective tissue clinically characterized by thickening and fibrosis of the skin and by distinctive forms of involvement of internal organs. One of the hallmarks of systemic sclerosis is the presence of serum autoantibodies against a variety of nuclear and cytoplasmic antigens. The primary purpose of this study was to identify the autoantibodies ...

Pediatric SLE (pSLE) is a chronic multisystem autoimmune disease with remitting, relapsing course and onset of symptoms before age 18 years, accounting for approximately 20% of all SLE. This clinically heterogeneous disease is characterized by a distinct spectrum of autoantibodies including antinuclear antibody (ANA), double-stranded deoxyribonucleic acid (dsDNA), and antibodies against extract...

A case of a 42-year-old female with hyperthyroidism was subsequently diagnosed to have systemic lupus erythematosus with distal RTA. The clinical examination on admission showed swelling of the knee joints and the urinalysis showed pH 6.5, pro 3+. Her blood routine results were as follows: white blood cells 1.85×109/L, platelets 100×109/L, erythrocyte 3.06×1012/L. The serum potassium was 3.11 m...

An 18-year-old male presented with a nonhealing wound on left lower limb, pain and swelling over multiple joints, weight loss, and yellowish discoloration of eyes and urine for the past 4 years. On examination, the patient had pallor, icterus, and generalized lymphadenopathy with a nonhealing unhealthy ulcer over left medial malleolus. He had deformed joints with hepatomegaly and splenomegaly. ...