Ambient ionization mass spectrometric imaging of all parts of the seedling of Dysoxylum binectariferum Hook. f (Meliaceae) was performed to reconstruct the molecular distribution of rohitukine (Rh) and related compounds. The species accumulates Rh, a prominent chromone alkaloid, in its seeds, fruits, and stem bark. Rh possesses anti-inflammatory, anti-cancer, and immuno-modulatory properties. D...

A human monoclonal anti-Rh(D) antibody produced by an Epstein-Barr virus (EBV)-transformed B-cell line (IgG1(lambda), clone H2D5D2) has been purified on protein A-Sepharose column and used for binding studies and immune precipitation of the blood group rhesus (Rh) antigens. Scatchard plot analyses show that the 125I-labeled antibody (iodo-gen procedure), binds to 1.09 X 10(5), 0.43 X 10(5), and...

Objective: To determine the incidence and the rate of red blood cell alloimmunization in polytransfused patients. Methods: A polytransfused patient was defined as having received at least 6 units of red cell concentrates during a 3-month period. The records of all patients (n = 12,904) who had received red blood cell units were examined retrospectively by searching the computer database at Hosp...

The discovery of ABO(H) blood group antigens resulted in the birth of transfusion medicine and allowed for the first time the use of a laboratory test that could predict immunologic outcomes prior to transfusion. Although ABO(H) antibody testing continues to represent one of the first and most common examples of personalized medicine, it was not until many years later that the existence of non-...

She was booked at about five weeks' gestation in a small general hospital and transferred to Royal Maternity Hospital, Belfast, at 24 weeks' gestation when quadruplet pregnancy was confirmed. She remained in hospital for six weeks and four days until delivery. There was no past medical history of note, blood group was O-Rh(D) negative and anti-D antibodies were detected (Table). The husband's b...

effect measurable lysis, and lysis increases linearly with increasing levels of antibody sensitization. 100 ,ug/ ml of IgGI abolished lysis even at maximal levels of anti-D sensitization (15,000 molecules/cell). Two isoimmune IgG anti-A or anti-B antisera were 5 to 10-fold less efficient in promoting phagocytosis or lysis per molecule of IgG bound; however, because of the greater antigen densit...