نتایج جستجو برای: anti peroxidase antibody

تعداد نتایج: 502114  

Journal: :Journal of clinical microbiology 1983
R E Bryant B N Chamovitz S A Morse M A Apicella V H Morthland

The specificity of the enzyme-linked immunosorbent assay(s) is thought to depend on the specificity of the antibody used in the assay system. Therefore, the association of broadly reactive antigens like endotoxin with enzyme conjugates or other enzyme-linked immunosorbent assay reagents has the potential of altering the specificity of reactions in the enzyme-linked immunosorbent assay. Using th...

Journal: :Clinical chemistry 1983
D J Litman R H Lee H J Jeong H K Tom S N Stiso N C Sizto E F Ullman

We describe an internally referenced immunochemical test-strip for use in the rapid detection of morphine. The method is based on the "enzyme-channelling" immunoassay technique, and a glucose oxidase-horseradish peroxidase enzyme pair is used to immunospecifically generate an insoluble, colored reaction product on the test-strip surface. Test strips are composed of two active surfaces, each of ...

Journal: :Journal of clinical pathology 1974
C R Taylor J Burns

A method is described for the demonstration of specific immunoglobulin in plasma cells and other lymphoid cells in sections taken from routine surgical histology specimens which have been formalin fixed and paraffin embedded. An indirect sandwich technique was employed using specific rabbit antihuman immunoglobulin antisera (anti-K, L, G, A, and M) and a swine antirabbit serum Ig G, conjugated ...

2013
Lizhi Liu Weiming Xu

The conjugates of monoclonal antibodies and nanoparticles, including quantum-dot(QD), offer significant advantages over conventional fluorescent probes to image and study biological processes. The extend stability, intense fluorescence and low toxicity of QDs are well suited for biological applications. In the present study, we used QD-conjugated anti-glucose-regulated protein 78(GRP78) antibod...

Journal: :The Yale Journal of Biology and Medicine 1982
J. F. Hsu A. S. Evans J. C. Niederman L. C. Cenabre

An enzyme-linked immunosorbent assay (ELISA) test has been developed for measurement of heterophile antibody. The microtiter test utilizes a bovine erythrocyte monolayer as antigen and anti-human IgM antiserum conjugated with horseradish peroxidase to measure the degree of binding of the heterophile antibody in the test serum with the erythrocytes. A single serum dilution yields quantitative re...

Journal: :Journal of neuroscience methods 1997
J Bhattacharjee B Nunes Cardozo W Kamphuis M Kamermans G F Vrensen

Immunodetection techniques are dependent on enzyme-protein conjugates for the visualisation of antigen-antibody complexes. One of the most widely used is the avidin-biotin-peroxidase complex (ABC) method. The present study demonstrates that direct treatment of goldfish and salamander retinal sections with ABC, followed by an incubation with the chromogenic substrate 3,3-diaminobenzidine tetrahy...

Journal: :Clinical chemistry 1981
A Turkes J Dyas G F Read D Riad-Fahmy

In this enzyme immunoassay for ethynyl estradiol, a conjugate of the 3-(O-carboxymethyl)ether with horseradish peroxidase is used as the label and a conjugate of the 6-(O-carboxymethyl)oxime with bovine serum albumin as the immunogen. A solid-phase antibody procedure is used in separating antibody-bound and free steroid. The lower limit of sensitivity of the assay is 2 pg per assay tube. Interf...

2011
Carol Lynam Niamh Gilmartin Richard O’Kennedy Gordon Wallace

The attachment of mouse immunoglobulin G (IgG) and anti-mouse IgG antibodies onto carbon nanotubes (CNTs), using either non-covalent or covalent means was investigated. The resultant CNTs were characterised using a variety of techniques including enzymelinked and fluorescence-linked immunoassays, UV-visible-NIR and Raman spectroscopy, transmission electron microscopy and cyclic voltammetry. TEM...

2003
Valentin Wittmann Sonja Seeberger

General. Solid-phase peptide synthesis was carried out in plastic syringe reactors. Fmocprotected amino acids were obtained from Novabiochem (Läufelfingen, Switzerland) or prepared according to standard procedures. Bovine serum albumin (A7638), biotinylated WGA (L5142), alkaline phosphatase-coupled monoclonal anti biotin antibody (A6561), BCIP/NBT tablets (B5655), peroxidase-labeled WGA (L7017)...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید