This experiment was conducted to investigate the effect of paclobutrazol as an anti-gibberellin on some morpho-physiological characteristics of Petunia × grandiflora ‘Bravo Blue’, and determine the optimum concentration of paclobutrazol for drought resistance in this plant. The experiment was carried out as factorial based on completely randomized design with three replications in greenhouse. T...

Gibberellins (GAs) are involved in the regulation of flowering and fruit-set in grapes (Vitis vinifera L.), but the molecular mechanisms behind this process are mostly unknown. In this work, the family of grapevine GA oxidases involved in the biosynthesis and deactivation of GAs was characterized. Six putative GA 20-oxidase (GA20ox), three GA 3-oxidase (GA3ox), and eight GA 2-oxidase (GA2ox) pr...

Barley endosperm halves release acid phosphatase in response to several gibberellins and gibberellin precursors. Seed halves incubated with 10(-7)m GA(3) at 29 degrees begin to release phosphatase after 11 hr and release it for another 26 hr in response to GA(3). After 37 hr, the rate of release slows to that of seed halves incubated without GA(3). GA(3) is active at 10(-10)m and maximally acti...

BACKGROUND AND PURPOSE NF-κB-driven inflammation is negatively regulated by the zinc finger protein A20. Gibberellic acid (GA3 ) is a plant-derived diterpenoid with documented anti-inflammatory activity, which is reported to induce A20-like zinc finger proteins in plants. Here, we sought to investigate the anti-inflammatory effect of GA3 in airway epithelial cells and determine if the anti-infl...

Bacteria have evolved gibberellin phytohormone biosynthesis independently of plants and fungi. Through 13C-labeling and NMR analysis, the mechanistically unusual "B" ring contraction catalyzed by a cytochrome P450 (CYP114), which is the committed step in gibberellin biosynthesis, was shown to occur via oxidative extrusion of carbon-7 from ent-kaurenoic acid in bacteria. This is identical to the...

Patatin-related phospholipase A (pPLA) hydrolyses glycerolipids to produce fatty acids and lysoglycerolipids. The Oryza sativa genome has 21 putative pPLAs that are grouped into five subfamilies. Overexpression of OspPLAIIIα resulted in a dwarf phenotype with decreased length of rice stems, roots, leaves, seeds, panicles, and seeds, whereas OspPLAIIIα-knockout plants had longer panicles and see...

Two fltnorene-9-carboxylic acid derivatives (fig 1, I and II) have recently been shown to be potent growth regulators in higher plants (1), and have been givein the trivial name, morphactins. The structtural similarity between the morphactins and gibberellic acid (fig 1, III) immediately suggested a common site of action. In fact, Ziegler, et al. (4) have claimed that methyl-morphactin (I) is a...