We investigated the regulation of organic anion transport driven by the organic anion transporter 3 (OAT3), a multispecific OAT localized at the basolateral membrane of the renal proximal tubule. PMA, a PKC activator, inhibited uptake of estrone sulfate (ES), a prototypic substrate for OAT3, in a dose- and time-dependent manner. This inhibition was reduced by 100 nM bisindoylmaleimide I (BIM), ...

Basolateral uptake of organic anions in renal proximal tubule cells is indirectly coupled to the Na(+) gradient through Na(+)-dicarboxylate cotransport and organic anion/dicarboxylate exchange. One member of the organic anion transporter (OAT) family, Oat1, is expressed in the proximal tubule and is an organic anion/dicarboxylate exchanger. However, a second organic anion carrier, Oat3, is also...

Giardia lamblia trophozoites were incubated for 2 h with activated murine macrophages, nitric oxide (NO) donors or a superoxide anion generator (20 mU/ml xanthine oxidase plus 1 mM xanthine). Activated macrophages were cytotoxic to Giardia trophozoites (approximately 60% dead trophozoites). The effect was inhibited (> 90%) by an NO synthase inhibitor (200 microM) and unaffected by superoxide di...

This paper studied the effect of pyridinium-based ionic liquids as cosolvents in a methanol-water solvent system on hydrolytic activity Candida rugosa lipase. These were successfully synthesized using imidazolium-based liquid synthesizing methods with certain adjustment. The lipase was analyzed 4-nitrophenol acetate (pNPA) and palmitate (pNPP) substrates. addition had no significant water solve...

it is very important to determine the stable nitrogen isotope ratio for the nitrogenous substances in aerosols and the geochemistry behavior of stable nitrate ion isotopes in aerosols in order to elucidate the chemical and physical functions of clouds. hence, aerosol field sampling was conducted in the skies offshore from malibu beach and santa barbara, california, u.s.a. on 16 and 18 july 200...

It has been shown in the previous publications’ that the eriect of neutral salts on gelatin is repressed in the presence of the salt and that we can study such effects with any profit only when the excess of salt is washed away after it has had a chance to act on the protein. Such gelatin reveals qualities which are masked in the presence of the salt and which allow us to gain a clear insight i...

The majority of mammalian cells demonstrate regulatory volume decrease (RVD) following swelling caused by hyposmotic exposure. A critical signal initiating RVD is activation of nucleotide receptors by ATP. Elevated extracellular ATP in response to cytotoxic cell swelling during pathological conditions also may initiate loss of taurine and other intracellular osmolytes via anion channels. This s...

The effects of adenosine were studied on human neutrophils with respect to their generation of superoxide anion, degranulation, and aggregation in response to soluble stimuli. Adenosine markedly inhibited superoxide anion generation by neutrophils stimulated with N-formyl methionyl leucyl phenylalanine (FMLP), concanavalin A (Con A), calcium ionophore A23187, and zymosan-treated serum; it inhib...

The presence of inorganic salts either as part the substrate or added to reaction medium are known significantly affect pathways during hydrothermal carbonisation (HTC) biomass. This work aims understand influence on by processing cellulose in one more with different valency. Batch experiments and Differential Scanning Calorimetry were used investigate change conversion. effect rate HTC can be ...