نتایج جستجو برای: and bphg genes cloned into pgreen vector were transformed into ecoli

تعداد نتایج: 17009540  

Azimi, Shohreh, Sohrabi, Nooshin,

Background and Objective: Leptospirosis as an important emerging infectious zoonotic disease caused by spirochetes of the genus Leptospira. Given the low sensitivity and long duration of its culture, the diagnosis of leptospirosis is mainly based on serological methods. The microscopic agglutination test (MAT) is considered as the reference method. Because of the complexity of the MAT, there is...

Journal: :jundishapur journal of microbiology 0
zahra sadat shahmoradi school of agriculture, shahid chamran university, ir iran +98-611333001115, [email protected] hamid rajabi memari school of agriculture, shahid chamran university, ir iran +98-611333001115, [email protected]; school of agriculture, shahid chamran university, ir iran +98-611333001115, [email protected] mojtaba khayyam nekoei agricultural biotechnology research institute of iran (abrii), ir iran daryoosh nabati ahmadi school of agriculture, shahid chamran university, ir iran +98-611333001115, [email protected] babak latif school of agriculture, tarbiat modares university, ir iran mehdi sharifitabar department of stem cells and developmental biology, cell science research center, royan institute, ir iran

background genetic manipulation of chloroplast in higher plants offers a number of unique prerogatives, including; undesirable of pleiotropic genome and gene silencing effects and also use as an important agronomic trait for producing essential biomaterials and industrial enzymes. in order to manipulate chloroplast genome, specific vectors are required. these vectors can be transformed and expr...

AH Ahmadi E Rahimi F Khani Habib abadi, M Behmanesh

Background Environmental and cellular inappropriate conditions can cause damages to cells nucleotide poll. Deamination and oxidation damages interfere with cell�s vital reactions. Inosine triphosphate pyrophosphatase (ITPA), an evolutionary conserved enzyme, plays a critical role in elimination of non-canonical bases. In human genome, the ITPA gene is located on chromosome 20 short arm and tran...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه سیستان و بلوچستان - دانشکده ادبیات و علوم انسانی 1392

abstract the present study was conducted in order to investigate the impact of an integrated model of form-focused and task-based instruction on iranian efl learners vocabulary learning and retention.it also aimed to detect efl learners attitude towards the implementation of form-focused task-based vocabulary instruction in the classroom. in order to address the purposes of this study, a sampl...

Journal: :iranian journal of virology 0
h hoseinian department of biology, islamic azad university, damghan branch, semnan, iran m moghbeli department of biology, islamic azad university, damghan branch, semnan, iran f behzadian department of molecular genetics, research center for sciences and biotechnology, malek ashtar university, tehran, iran

background and aims: the ectodomain of matrix protein of influenza virus is a weak immunogen that is highly conserved among all subtypes of influenza a virus. tandem repeats of these genes along with linker were used to enhance immunogenicity of m2e protein and so it can be served as a universal vaccine in both humans and livestock. materials and methods: in this study, the sequences of extra-d...

Journal: :iranian journal of biotechnology 2015
jinyang zhang zian jin tao sun yan jiang qinqin han

background: rabies virus (rabv) is a deadly neurotropic virus that causes the disease of rabies in humans and animals. l protein is one of the large structural protein of rabies virus, which displays multiple enzymatic activities, and is required for viral transcription and replication.objectives: a truncated l protein of rabies virus is being cloned, expressed and purified to produce relevant ...

Abbas Sahebghadam Lotfi, Behzad Adibi Mahboobe Ghaedi Masoud Soleimani, Mehdi Shamsara Sare Arjmand

In this study, in order to facilitate and accelerate the production of eukaryotic protein alpha 1-antitrypsin (AAT) with correct post-translational modifications, a protein production system based on the transduction of CHO and COS-7 cells using lentiviral vectors was developed. Human AAT cDNA was cloned into a replication-defective lentiviral vector. The transgene AAT-Jred chimer was transferr...

Journal: :iranian journal of parasitology 0
vahid nasiri abdolhossein dalimi fatemeh ghaffarifar azam bolhassani

background: the aim of present study was to evaluate the protective efficacy of live recombinant l. tarentolae expressing kmp11-ntgp96-gfp fusion as candidates for live engineered recombinant vaccine against visceral leishmaniasis in balb/c mice. methods: kmp-11 and nt-gp96 genes cloned into the pjet1.2/blunt cloning vector and then into pegfp-n1 expression vector. the kmp-11, nt-gp96 and gfp f...

پایان نامه :0 1374

the research questions were as follows: 1. is there any relationship between the students concious awareness of the form and implications of the conjuncations and their improvement in using appropriate conjunctions? 2. does students knowledge of the from and the implications of the conjunctions help them to produce more coherent writings. 3. does a comparison between english conjunctions and th...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شهید باهنر کرمان - دانشکده ادبیات و علوم انسانی 1392

abstract this study aimed at investigating the translation of interjections from english into persian in dubbing. to carry out this study the expressive secondary interjections found in the film four weddings and a funeral and their equivalent in persian dubbed version were analyzed through the strategies proposed by cuenca. the descriptive analysis of the corpus showed that (strategy b) i.e...

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