Although somatically mutated autoantibodies are characteristic of many autoimmune diseases, the processes that can lead to their development remain poorly understood. We have examined the formation of autoreactive memory B cells in PevHA mice, which express the influenza virus PR8 hemagglutinin (HA) as a transgenic membrane bound neo-self-Ag. Using a virus immunization strategy, we show that PR...

Transgenic expression of the influenza virus hemagglutinin (HA) in the pancreatic islet beta cells of InsHA mice leads to peripheral tolerance of HA-specific T cells. To examine the onset of tolerance, InsHA mice were immunized with influenza virus A/PR/8 at different ages, and the presence of nontolerant T cells was determined by the induction of autoimmune diabetes. The data revealed a neonat...

Minor histocompatibility Ags (mHags) are important targets of the graft-versus-leukemia effect after HLA-matched allogeneic stem cell transplantation. mHags are HLA-restricted polymorphic peptides expressed on normal and leukemia cells. Vaccination with hematopoiesis-restricted mHag peptides, such as HA-1, may boost the graft-versus-leukemia effect. However, some animal studies indicate that pe...

BACKGROUND ATP-binding cassette transporter A1 (ABCA1) is a membrane integral protein which plays a vital role in High Density Lipoprotein (HDL) metabolism and exerts a protective effect against Hypoalphalipoproteinemia (HA) by mediation of rate-limiting step in HDL biogenesis. In addition, this protein possesses anti-inflammatory effects by inhibiting the production of some inflammatory cytoki...

The conservation technologies and traditional techniques were evaluated at Adaptive Research Farm Gujranwala Pakistan during Rabi 2007-2008 and 2008-2009. The sowing techniques evaluated were broadcasting (T-1), Rotary Tillage Drill (T-2), Zero Tillage Drill (T-3) and Rabi Drill (T-4). Rotary Tillage Drill showed significantly high germination count (220.50 m -2 ) followed by Rabi Drill (211.50...

Optimization of long term immunity depends on the functional persistence of memory T cells; however, there are no defined strategies for promoting memory T cell function and survival. In this study, we hypothesized that varying the strength of the recall stimulus could modulate the function and survival potential of memory CD4 T cells. We tested the ability of peptide variants of influenza hema...

the objective of this study was to evaluate the effect of different levels of irrigation and nitrogen fertilizer on nitrate movement, yield, and water use efficiency (wp) of corn in no-till planting method. the research was conducted in two years as a randomized complete block design arranged in a split block treatment design with three replicates in the safiabad agricultural research center. i...

Unprimed T cells transferred to heavily irradiated H-2-compatible mice cause a high incidence of lethal graft-vs.-host disease (GVHD)a in certain strain combinations (1-3). GVHD in this setting is a consequence of mature post-thymic donor T cells responding to the multiple minor histocompatibility antigen (minor HA) differences of the host. With the combination of CBA and B10.BR (both H-2~), do...

Adsorption of Bovine Serum Albumin (BSA) onto calcium Hydroxyl Apatite (HA), Ca10PO4(OH)2, has been studied by Ultra Violet (UV) spectroscopy. The adsorption isotherms of BSA onto the HA surface at 291.7 K and 303.2 K were satisfactorily presented by Langmuir equation and the evaluated parameters are reported. The specific surface area of H...

Nonobese diabetic (NOD) mice develop spontaneous autoimmune diabetes that involves participation of both CD4+ and CD8+ T cells. Previous studies have demonstrated spontaneous reactivity to self-Ags within the CD4+ T cell compartment in this strain. Whether CD8+ T cells in NOD mice achieve and maintain tolerance to self-Ags has not previously been evaluated. To investigate this issue, we have as...