the objective of this research was to study the feasibility of a two stage continuous system employing anaerobic-aerobic microbial film for domestic wastewater treatment and the effect of iron on the behavior of sulfate reducing bacteria in anaerobic metabolism. a bench scale system with an anaerobic filter followed by aerobic fixed units used plastic media and was operated in up flow manner wi...

A newly isolated denitrifying bacterium, Thauera sp. strain DNT-1, grew on toluene as the sole carbon and energy source under both aerobic and anaerobic conditions. When this strain was cultivated under oxygen-limiting conditions with nitrate, first toluene was degraded as oxygen was consumed, while later toluene was degraded as nitrate was reduced. Biochemical observations indicated that initi...

Membrane-aerated biofilm reactors performing autotrophic nitrogen removal can be successfully applied to treat concentrated nitrogen streams. However, their process performance is seriously hampered by the growth of nitrite oxidizing bacteria (NOB). In this work we document how sequential aeration can bring the rapid and long-term suppression of NOB and the onset of the activity of anaerobic am...

The presence and activity of denitrifying bacteria as well as bacteria capable of reducing sulfate in 1 upland and 5 riparian soils of a mountain watershed in Wyoming were studied. Bacteria were enumerated from soil samples collected during summer along transects placed perpendicular to stream flow. Samples were taken at 3 depths within each plant community. Subsamples were frozen and later uti...

Antibiotics and antibiotic resistance genes have shown to be omnipresent in the environment. In this study, we investigated the effect of vancomycin (VA) on denitrifying bacteria in river sediments of a Waste Water Treatment Plant, receiving both domestic and hospital waste. We exposed these sediments continuously in flow-through reactors to different VA concentrations under denitrifying condit...

Recent studies of anaerobic toluene catabolism have demonstrated a novel reaction for anaerobic hydrocarbon activation: the addition of the methyl carbon of toluene to fumarate to form benzylsuccinate. In vitro studies of the anaerobic benzylsuccinate synthase reaction indicate that the H atom abstracted from the toluene methyl group during addition to fumarate is retained in the succinyl moiet...

Denitrifying organisms are essential in removing fixed nitrogen pollutants from ecosystems (e.g. sewage sludge). They can be detrimental (e.g. for agricultural soil) and can also produce the greenhouse gas N2O (nitrous oxide). Therefore a more comprehensive understanding of this process has become increasingly important regarding its global environmental impact. Even though bacterial genome seq...

Low air pressure is a feature of high-altitude regions. Domestic wastewater from such regions typically has low carbon-to-nitrogen ratio (C/N ratio). These factors combine to make traditional biological nitrogen removal in inefficient and more energy-intensive. The partial denitrification-anaerobic ammonium oxidation (PD/A) process was reported remove ammonia municipal sewage, consuming fewer c...