The planned upscaling of vector control strategies requires insight into the epidemiological consequences of vector resistance. Therefore, the pyrethroid and DDT resistance status of Anopheles gambiae s.l. was assessed in Uganda from 2004 to 2006, and spatial and seasonal variations in knockdown resistance (kdr) frequencies were analyzed in terms of epidemiological significance. Anopheles gambi...

We investigated the frequencies of single and multiple matings in field-collected female Anopheles gambiae by conducting microsatellite DNA analyses on the sperm contained within their spermatheca. Amplifcation by a polymerase chain reaction (PCR) at four loci allowed the detection of sperm extracts exhibiting more than two alleles per locus, thereby revealing the occurrence of multiple insemin...

Population subgroups of the African malaria vector Anopheles gambiae have not been comprehensively characterized owing to the lack of unbiased sampling methods. In the arid savanna zone of West Africa, where potential oviposition sites are scarce, widespread collection from larval pools in the peridomestic human habitat yielded a comprehensive genetic survey of local A. gambiae population subgr...

Effective population size (Ne) is a single number which allows us to relate a large part of population-genetics theory built around ideal populations to real populations. In the case of Anopheles gambiae, Ne is important to interpreting the temporal and spatial distribution of genes. These patterns are in turn used to explore the structure of (typically non-ideal) natural populations. We discus...

This study was carried out between May and October, 2010 to morphologically identify Anopheles species in parts of Kano State. A total of 2374 Anopheles were collected and identified. 1782 (75.07%) were collected from Nassarawa Local Government Area while 592 (24.93%) were collected from Tarauni Local Government Area. Using Anopheles characters of Gilles and Coetzee (1987) under zeiss light mic...

Plasmodium falciparum is the causative agent of malaria, a disease that kills almost one million persons each year, mainly in sub-Saharan Africa. P. falciparum is transmitted to the human host by the bite of an Anopheles female mosquito, and Anopheles gambiae sensus stricto is the most tremendous malaria vector in Africa, widespread throughout the afro-tropical belt. An. gambiae s.s. is subdivi...

This study in Senegal compared the feeding preferences of Anopheles gambiae and A. arabiensis while controlling for equal accessibility to hosts located outdoors under bed net traps. All fed A. gambiae complex females were identified with the aid of the polymerase chain reaction and their blood meal sources were identified by enzyme-linked immunosorbent assay. A total of 605 anophelines, includ...

BACKGROUND Vector control is one of the most effective measures to prevent the transmission of malaria, a disease that causes over 600,000 deaths annually. Around 30-40 Anopheles mosquito species are natural vectors of malaria parasites. Some of these species cannot be morphologically distinguished, but have behavioral and ecological differences. Emblematic of this is the Anopheles gambiae spec...

Malaria control programmes in Africa, for the most part, address only treatment of the disease and supply of insecticide treated bed nets. The impact of these restricted programmes has been limited and new approaches are being advocated, including integrated vector management strategies and partnerships with industry. Mosquito surveys were carried out for AngloGold/Ashanti in preparation for th...

Haematophagous insects are frequently carriers of parasitic diseases, including malaria. The mosquito Anopheles gambiae is the major vector of malaria in sub-Saharan Africa and is thus responsible for thousands of deaths daily. Although the role of olfaction in A. gambiae host detection has been demonstrated, little is known about the combinations of ligands and odorant binding proteins (OBPs) ...