We investigated the phenomena of antimicrobial peptides (AMPs) directly attacking the cytoplasmic membranes of Escherichia coli spheroplasts. We developed a procedure for fluorescence recovery after photobleaching to examine dye leakage through bacterial membranes as AMPs in solution bound to the membranes. We found that the AMP binding did not increase the apparent membrane area of a spheropla...

The availability of simple, robust, and cost-effective methods for the large-scale production of bacteriotoxic peptides such as antimicrobial peptides (AMPs) is essential for basic and pharmaceutical research. However, the production of bacteriotoxic proteins has been difficult due to a high degree of toxicity in bacteria and proteolytic degradation. In this study, we inserted AMPs into the Gre...

Presently, many studies have focused on exploring in silico approaches in the identification and development of alternative therapy for the treatment and management of cancer. Solute carrier family-2-member-4-gene (Slc2a4) which encodes glucose transporter 4 protein (GLUT4), has been identified as a promising therapeutic target for cancer. Though Slc2a4 is known to play a major regulatory role ...

A novel adsorbent (AMPS-silica) was synthesized by bounding AMPS (2-acrylamido-2-methylpropanesulfonic acid) onto silica surface, which functioned with y-methacryloxypropyltrimethoxysilane reagent. The adsorbent was characterized by nitrogen adsorption/desorption measurement, thermogravimetric analysis (TGA) and potentiometric titration analysis. The TGA result indicated that the surface modifi...

Antimicrobial peptides (AMPs) are small, endogenous, polycationic molecules that constitute a ubiquitous and significant component of innate immunity. These natural antibiotics have broad microbicidal activity against various bacteria, fungi, and enveloped viruses. Because most AMPs kill bacteria by physical disruption of cell membranes, which may prevent microorganisms from developing resistan...

Antimicrobial polypeptides (AMPs) are effector molecules of the innate immune defense. AMPs are mainly expressed in epithelial cells and immune cells, providing the first line of defense to infection as direct antimicrobials. In addition, many AMPs display immunomodulatory functions in both the adaptive and innate immune system. Thus, a tight control of AMP-expression is necessary for a functio...

New treatments based on peripheral stimulation of the sensory-motor system have been inspiring new rehabilitation approaches in Parkinson's disease (PD), especially to reduce gait impairment, levodopa washout effects, and the incidence of falls. The aim of this study was to evaluate the change in gait and the clinical status of PD patients after six sessions of a treatment based on automated me...

This paper demonstrates the enrichment of reticulocytes by centrifuging whole blood through aqueous multiphase systems (AMPSs)-immiscible phases of solutions of polymers that form step-gradients in density. The interfaces of an AMPS concentrate cells; this concentration facilitates the extraction of blood enriched for reticulocytes. AMPS enrich reticulocytes from blood from both healthy and hem...

To provide an acceptable call blocking probability in circuit-switched cellular networks, such as the Advanced Mobile Phone System (AMPS) networks, a significant fraction of the channel capacity in each cell is normally unused. This “free” capacity can be effectively used for packet data transmissions that yield to voice traffic when necessary. Cellular Digital Packet Data (CDPD) is a packet-sw...