A screen printed carbon electrode (SPCE) containing the electrocatalyst Meldola’s Blue (MB) has been investigated as the base transducer for a glutamate biosensor. The sandwich biosensor was fabricated by firstly depositing a chitosan (CHIT) layer onto the surface of the transducer (MB-SPCE), followed by glutamate dehydrogenase (GLDH): this device is designated GLDH-CHIT-MB-SPCE. NAD was added ...

The human dopamine transporter (hDAT) regulates synaptic dopamine (DA) levels and is the site of action of abused and therapeutic drugs. Here we study the effect of a threonine residue (Thr62 in hDAT) that is highly conserved within a canonical phosphorylation site (RETW) in the juxtamembrane N-terminal region of monoamine transporters. In stably transfected human embryonic kidney 293T cells, e...

The dopaminergic system has been suggested to serve as a functional target for ketamine, especially with its addictive and antidepressant effects. In this work, intracellular vesicle impact electrochemical cytometry single cell amperometry were applied model line show that the intravesicular contents number of exocytotic events increased fraction release decreased after treatment by subanesthet...

The trivalent Eu(III) ion exhibits unique red luminescence and plays an significant role in the display industry. Herein, amperometry electrodeposition method was employed to electrodeposit materials on porous Si terpyridine-functionalized surfaces. electrodeposited were fully characterized by scanning electron microscopy, X-ray diffraction crystallography, Fourier-transform infrared spectrosco...

A glassy carbon electrode was modified with graphene oxide nanosheets and a hybrid of copper-cobalt hexacyanoferrate. The nanocomposite was characterized by cyclic voltammetry, FT-IR and scanning electron microscopy. Cyclic voltammetry showed a stable and reversible redox pair with surface confined characteristics in phosphate buffer solution (0.1 M, pH 3). Hydrodynamic amperometry was used for...

A reversed-phase liquid chromatographic separation with pulsed amperometric detection of phenolic acids at a glassy carbon electrode is described. Chromatographic separation was carried out in isocratic conditions using 0.20 mol·L-1 acetic acid (pH 5.0)/water (80 : 20, v/v) as mobile phase under constant working potential mode of 0.80 V. Chromatographic peaks presented high resolution and separ...

This short review is aimed at emphasizing the recent most prominent advances in electrochemical investigation of interactions molecules with purely lipid bilayers. The first part which deals electrode-supported bilayer membranes articulated around nature electrode material a focus on mercury to celebrate this special issue entitled “100 years from polarography contemporary electrochemistry”. se...