PURPOSE To describe the phenotypic characteristics of a limbal epithelial cell sheet outgrowth from a limbal explant cultured on amniotic membrane. METHOD Immunofluorescent staining and confocal microscopy were used to examine the expressions of p63, Ki-67, keratins 3 and 14, connexin 43, and the integrin alpha6/beta4 and alpha3/beta1 subunits in corneal and limbal tissues in a limbal explant...

BACKGROUND AIMS Amniotic membrane (AM), the innermost layer of human placenta, is composed of a single layer of epithelial cells, a basement membrane and an avascular stroma. The AM has many functions and properties, among which angiogenic modulatory and immunoregulatory effects are applicable in cancer therapy. Because these functions belong to amniotic epithelial cells, in this study we compa...

AIMS To evaluate the role of amniotic membrane transplantation in patients with acute ocular burns. METHODS In a prospective, randomised, controlled clinical trial, 100 patients with grade II to IV acute ocular burns (Roper Hall Classification) were recruited. 50 patients with grade II-III burns were graded as moderate burns, and 50 patients with grade IV burns were graded as severe burns. Bo...

Dear Editor,I read with interest the article on outpatient management of burn wounds published in a recent issue of the IJMS.1 There are many questions that arise which are worth mentioning. Under the methodology section, no description was provided on how patients were randomized into the two treatment arms and, therefore, it is not possible to assess how confounding variables were controlled....

There has been a great attention to the capacities of the amniotic membrane and its stem cells in the recent years. This membrane has been shown to have several unique capacities such as antitumor effects [1,2] as well as angiogenesis and vascular formation capacities [3]. However it is important to notice that in order to consider the amniotic membrane as a feasible option that is practical fo...

Submit Manuscript | http://medcraveonline.com Abbreviations: AM: Amniotic Membrane; MO: Moringa Oleifera; ITBBR: Institute of Tissue Banking and Biomaterial Research; VEGF: Vascular Endothelial Growth Factor; HGF: Hepatocyte Growth Factor; TGF: Transforming Growth Factor; HLA: Human Leukocyte Antigen; DR: Differential Regulation; MSC: Mesenchymal Stem Cells; SLPI: Secretary Leukocyte Proteinase...

BACKGROUND To observe the impact of application of bio-amniotic membrane immersed in 5-fluorouracil solution in trabeculectomy on the retina in a rabbit model. MATERIALS AND METHODS Healthy white New Zealand rabbits were randomly assigned into three groups with 20 in each group. Bio-amniotic membranes of 4 × 5 mm immersed in either physiological saline/water for 10 min, or 25 mg/mL 5-fluorour...

background: amniotic membrane derived stem cells (amscs) have considerable advantages to use in regenerative medicine and their anti- inflammatory effects, growth factor secretion and differentiation potential make them suitable candidates for stem cell therapy of nervous system. the developing and neonatal brain contains a spectrum of growth factors to direct development of endogenous and dono...

BACKGROUND Mesenchymal stem cells (MSCs) are ideal candidates for treatment of diseases. Amniotic membranes are an inexpensive source of MSCs (AM-MSC) without any donor site morbidity in cell therapy. Adipose tissue derived stem cells (ASCs) are also suitable cells for cell therapy. There is discrepancy in CD271 expression among MSCs from different sources. In this study, the characteristics of...