an experiment was conducted to reevaluate the digestible lysine requirement of arian male broilers by comparing the performance of chicks fed different levels of cottonseed meal (csm) on a total amino acid (aa) versus a digestible aa basis. four hundred and thirty two (432) arian male broiler chicks were allotted to 18 treatments with four repli-cates of six chicks each in a completely randomiz...

A cDNA clone encoding a taste-modifying protein, miraculin (MIR), was isolated and sequenced. The encoded precursor to MIR was composed of 220 amino acid (aa) residues, including a possible signal sequence of 29 aa. Northern blot analysis showed that the mRNA encoding MIR was already expressed in fruits of Richadella dulcifica at 3 weeks after pollination and was present specifically in the pulp.

Quantitative traits locus for milk production traits has been described on centromeric end of bovine chromosome 14. Reports name the acyl coA: diacylglycerol acyltransferase (DGAT1) gene as a potential candidate gene with dinucleotide substitution (AA to GC) in exon VIII which causes the change of lysine to alanine in amino acid (K232A).The aim of the present study was to estimate the frequency...

Neuropeptide hormones produced by neurosecretory cells in the central or peripheral nervous systems regulate various physiological and behavioral events during insect development and reproduction. PBAN/Pyrokinin is a major neuropeptide family, characterized by a 5-amino-acid C-terminal sequence, FXPRLamide. This family of peptides has been implicated in regulating various physiological function...

Octahedral chiral-at-metal Ir(III) complexes exhibit excellent structural stability and stereoselectivity in asymmetric synthesis. Selectively oxidative dehydrogenation of amino acids could be achieved by exploiting such as chiral templates. The obtaining stable imine can then utilized nucleophilic additions to generate corresponding amine compounds. In this study, a conveniently synthesized [Λ...

Reversed-phase high performance liquid chromatographic (RPHPLC) determination of amino-acids with on-line pre-column ortho-phthalaldehyde (OPA) derivatization and fluorescence detection is rapid and sensitive. However, high-performance ionexchange chromatography (HP-IEC) with post-column ninhydrine reaction is the most widely used amino-acid (AA) assay for biological samples. These two methods ...

In cytopathic bovine viral diarrhea virus genotype 1 (BVDV1) isolates, insertions are reported at position A (amino acid [aa] 1535) and position B (aa 1589). Insertions at position B predominate. In this survey it was found that in BVDV2, insertions at position A predominate. Possible reasons for this difference in relative frequency are discussed.

The aim of this study was to investigate the amino acid (AA) release from breads enrichment with edible insects, Alphitobius diaperinus and Tenebrio molitor or pea protein during in vitro gastrointestinal digestion. Bread enriched at 5 10% insect flour protein. Enriched control were subjected standardised static free AAs before after each phase digestion (gastric, intestinal end digestion) dete...

Imiquimod (IMQ), a TLR7 agonist, is standard local treatment for non-melanoma skin cancers (NMSC). IMQ triggers inflammation, ultimately resulting in immunological tumor destruction. Albeit promotes the recruitment of effector T (TE) cells, anergy CD4+ cells. Meanwhile, how affects human CD8+ TE pivotal to cancer control, remains unclear. To address this, we studied cell lines from NMSC and cir...

We have determined the molecular basis for hypoxanthine-guanine phosphoribosyltransferase (HPRT) deficiency in a patient, J.H., with Lesch-Nyhan syndrome. Radioimmunoassay of lysates of erythrocytes or cultured B-lymphoblasts showed that this patient had no detectable HPRT enzyme activity or HPRT protein. HPRT-specific mRNA levels were normal by Northern analysis. We created a cDNA library from...