The extracellular matrix protein BM-40 (osteonectin, SPARC) has recently been shown to be a major target for transglutaminase-catalyzed cross-linking in differentiating cartilage. In the present study we demonstrate that recombinant human BM-40 can be modified with [3H]putrescine in a 1:1 molar ratio by transglutaminaseC (tissue transglutaminase). Residues Gln3 and Gln4 were identified as major...

Biochar-based carbonaceous adsorbents are gaining interest due to their high availability, ease of modification, and low cost; however, they show limited adsorption CO2 in flue gas common textural properties. In this study, TEPA-modified biochar was used prepare a solid amine adsorbent for the efficient capture gas. First, porous prepared with FeCl3, Mg(NO3)2, H2O (g) as activators walnut shell...

The aza-Michael addition is a versatile reaction for the modification of ?,?-unsaturated carbonyl compounds with amines. reactivity dimethyl itaconate as bio-based Michael acceptor explored in this work. Through its reactions piperidine and dibutylamine, it was found that order can be changed by choice catalyst, solvent, or concentration amine reactant. effectiveness catalysts proportional to t...

Post-translational modification (PTM) modulates and supplements protein functionality. In nature this high precision event requires specific motifs and/or associated modification machinery. To overcome the inherent complexity that hinders PTM's wider use, we have utilized a non-native biocompatible Click chemistry approach to site-specifically modify TEM β-lactamase that adds new functionality....

Post-translational modification (PTM) modulates and supplements protein functionality. In nature this high precision event requires specific motifs and/or associated modification machinery. To overcome the inherent complexity that hinders PTM's wider use, we have utilized a non-native biocompatible Click chemistry approach to site-specifically modify TEM b-lactamase that adds new functionality....

Biodegradable cationic poly(β-amino esters) (PBAE) nanoparticles are promising tools for delivering genes into various types of cells and tissues. Specific end-modification of the PBAE terminal parts significantly improves the efficiency of gene delivery in vitro and in vivo, and reduces cytotoxicity. Here, we demonstrated that amine end-modified PBAE nanoparticles can be used for intradermal d...

The electrochemical and chemical modification of carbon by reduction of diazonium salts attracted a lot of interest in the past decade. The major disadvantage of the modification approach based on aryl diazonium salt is that the synthesis of the salt is required. Indeed, the synthesis and isolation of the diazonium salt are not always straightforward. The synthesis is commonly carried out in ac...

in order to increase the rate of gas absorption, chemical reaction in the liquid phase is considered. the purpose of this paper was to simulate gas absorption process by a liquid film over a spherical packing incorporating eddy viscosity and diffusivity. second order chemical reaction for two different cases (fast and slow) were considered. the system partial differential equations obtained was...

Transglutaminases (TGs) are a family of enzymes that catalyze Ca(2+)-dependent post-translational modification of proteins by introducing protein-protein crosslinks (between specific glutamine and lysine residues), amine incorporation, and site-specific deamidation. In this study, new amine acceptor protein substrates of TG were isolated from rat liver extract and identified using 5-(biotinamid...

The nitrogen mustards used in these experiments were the hydrochlorides of ethyl bis (/3 chloroethyl) amine, methyl bis (/3 chloroethyl) amine, and tris (fl chloroethyI) amine. Sodium nucleate was prepared from calf thymus by a modification of Hammarsten's procedure (5). After the preliminary extraction, filtration, and addition of CaC12, a precipitate was obtained by bringing the final ethanol...