Human glucuronate 2-sulphatase (GAS), which is involved in the degradation of the glycosaminoglycans heparan sulphate and chondroitin 6-sulphate, was purified almost 2000000-fold to homogeneity in 8 0 yield from liver with a four-step six-column procedure, which consists of a concanavalin A-Sepharose/Blue A-agarose coupled step, a DEAE-Sephacel/octyl-Sepharose coupled step, CM-Sepharose chromat...

A heparin-binding protein was isolated from bovine uteri and purified to homogeneity. This protein appears as a double band of approx. 78 kDa in SDS/polyacrylamide-gel electrophoresis and has an isoelectric point of 5.2. The binding of heparin to this protein is saturable. No other glycosaminoglycan from mammalian tissue, such as hyaluronic acid, chondroitin sulphate, dermatan sulphate or kerat...

Chondroitin sulphate proteoglycans are expressed in a temporally restricted pattern from embryonic day 17 to postnatal day 0 in both the thalamus and the cortical subplate, to which thalamic neurones transiently project. To study whether chondroitin sulphate proteoglycans could be specifically involved in the modulation of thalamic axon outgrowth, we compared neurite outgrowth from cultured rat...

Mean serum concentrations of oestradiol-17beta, oestrone, and oestrone sulphate in postmenopausal women were the same when measured up to six hours after treatment with either piperazine oestrone sulphate 1.5 mg or oestradiol valerate 2 mg. Maximum concentrations of oestradiol were less than those of oestrone, but oestrone sulphate reached concentrations about 30 times higher than those of oest...

We have evaluated the possible role of proteoglycans in the uptake of spermine by human lung fibroblasts. Exogenous glycosaminoglycans behaved as competitive inhibitors of spermine uptake, the most efficient being heparan sulphate (Ki=0.16+/-0.04 microM). Treatment of fibroblasts with either heparan sulphate lyase, p-nitrophenyl-O-beta-D-xylopyranoside or chlorate reduced spermine uptake consid...

the sulphated form of ascorbic acid may play a role in the maintenance of connective tissue, suggested that although 35S-labelled glycosaminoglycans are formed in the presence of ascorbic acid 2-[35S]sulphate, they are not formed by direct transfer of the sulphate moiety from ascorbic acid 2-[35S]sulphate but by a decomposition product of the ester (Shapiro & Poon, 1975). The role of ascorbic a...

Background: Eclampsia is a hypertensive disorder related to pregnancy, in which there is occurrence of one or more generalized convulsions and or coma, in the absence of other neurologic conditions. It is a common obstetric emergency. Eclampsia is associated with elevated maternal and fetal morbidity and mortality. Objectives: To compare the efficacy of low dose magnesium sulphate (Dhaka) regim...

s-The uronic acid-containing glycosaminoglycans present in the brains of rat, monkey, chicken, sheep and rabbit were isolated into various fractions by combining the cetyl pyridinium procedure and DEAE-Sephadex column Chromatography. The analyses of the fractions show that hyaluronic acid, chondroitin4sulphate, chondroitin-6-sulphate, heparan sulphate and a testicular hyaluronidase-resistant ga...

introduction: controlling parenchymal hemorrhage, especially in liver, is still one of the challenges surgeons face with when they try to save the patients’ lives despite improvements in surgical procedures. there is a research contest between the researchers in this field to introduce more effective methods. this study aimed to compare the hemostatic effect of ferric sulfate and ferric chlorid...