Background In tissue engineering, scaffold characteristics play an important role in the biological interactions between cells and the scaffold. Cell adhesion, proliferation, and activation depend on material properties used for the fabrication of scaffolds. Objective In the present investigation, we used collagen with proper characteristics including mechanically stability, biodegradability ...

Tuberculous Pleural effusion is one of the most frequent causes of exudates in large series of pleural effusions in immunocompetent patients. The Aim of this study is to estimate enzyme Alkaline Phosphatase levels in serum and pleural fluids of HIV seronegative exudative Tuberculous Pleural fluids and their possible role in the diagnosis of tubercular pleural effusion. Serum and Pleural fluid s...

A standard assay for measuring alkaline phosphatase activity is detecting the production of nitrophenol from the artificial substrate p-nitrophenol phosphate. Nitrophenol absorbs light at 420 nm, which provides a convenient detection method. However, the absorbance of nitrophenol varies with pH. This variation complicates the use of the colorimetric assay to study the pH-dependence of alkaline ...

The primary, secondary, and final reaction products of the cytochemical staining method for alkaline phosphatase have been demonstrated by x-ray emission microanalysis. The advantages and limitations of the technique in measuring reaction products are discussed. Scanning x-ray emission analysis provides a rapid way of detecting and measuring the amounts of individual elements in cytochemical re...

Approximately 10% of the alkaline phosphatase activity in human kidney is derived from the intestinal-type alkaline phosphatase isoform, which can be differentiated from adult intestinal alkaline phosphatase by selective reactivity with monoclonal antibodies. The NH2-terminal sequence of the renal intestinal-type alkaline phosphatase was shown to be identical to sequences of the adult and mecon...

AIM To determine the cellular profile of human chronic periradicular lesions using immunohistochemical methods in order to study the differences in the cell infiltrate of periradicular granulomas and cysts. METHODOLOGY The study population consisted of 45 individuals without any systemic disease. Biopsies were obtained during periradicular surgery. Paraffin-embedded sections were stained by t...

The gene encoding Thermus caldophilus GK24 (Tca) alkaline phosphatase was cloned into Escherichia coli. The primary structure of Tca alkaline phosphatase was deduced from its nucleotide sequence. The Tca alkaline phosphatase precursor, including the signal peptide sequence, was comprised of 501 amino acid residues. Its molecular mass was determined to be 54¿ omitted¿760 Da. On the alignment of ...

Two members of a placental alkaline phosphatase (PLAP) family, PLAP and PLAP-like or germ cell alkaline phosphatase, are aberrantly expressed in tumors of ecotropic origin. To characterize alkaline phosphatase induced in seminoma, alkaline phosphatase cDNA clones were isolated from a cDNA library constructed from seminoma cells and characterized by nucleotide sequence determination. Thus, isola...