Glycogen synthetase b has been purified about 2,000-fold from a crude extract of rat liver. The purification procedure consisted of the separation of the glycogen pellet by centrifugation, solubilization of the synthetase by phosphorolysis of the glycogen by endogenous phosphorylase, and adsorption and elution from Ca3(P04)2 gel. The final material appears homogeneous on gel electrophoresis. A ...

The Raman and infrared (IR) spectra for the alkali metaphosphate crystals composed of Q2 units pyrophosphate crystal Q1 were assessed by density functional theory calculations. assignments peaks calculated IR consistent with reported phosphate glasses except assignment around 1100 cm?1 crystals. Peaks have been assigned to asymmetric stretching vibrations bonds between phosphorus atoms non-brid...

RNA, the earliest genetic and catalytic molecule, has a relatively delicate and labile chemical structure, when compared to DNA. It is prone to be damaged by alkali, heat, nucleases, or stress conditions. One mechanism to protect RNA or DNA from damage is through site-specific methylation. Here, we propose that RNA methylation began prior to DNA methylation in the early forms of life evolving o...

A new technique is described for studying controlled in vitro extension of plant cell walls. This technique uses frozen-thawed Avena coleoptile segments in which turgor is replaced by a constant applied force. In this system rapid extension is induced by low pH (3.0-3.6) but not by auxins or CO(2). Extensions of over 30% have been achieved. Our results indicate that cell wall synthesis is not r...

1 . Introduction The rejoining of DNA single-strand breaks in mammalian cellular DNA has been varified for cells in culture (e .g ., Alexander, Dean, Lehmann, Ormerod, Feldschreiber and Serianni 1970, Donlon and Norman 1971) as well as for ascites tumour cells in mice (Matsudaira, Nakagawa and Hishizawa 1969), and mouse testis (Ono and Okada 1974) and dog neurons (Wheeler and Lett 1972) irradia...

We have shown that a soluble, low-molecular weight, alkali-labile and oxidation-sensitive factor, prepared from the electric organ of Torpedo ocellata, induces Cap-dependent acetylcholine (ACh) release from isolated Torpedo synaptic vesicles [ 1,2]. Diverse activities of ascorbic acid in the brain have been reported [3-81. These include effects on metabolism of biogenic amines [9] and on releas...

We report pK(a) values with measurement uncertainties for all labile protons of the 27 tri-peptides prepared from the amino acids glutamic acid (E), glycine (G) and histidine (H). Each tri-peptide (GGG, GGE, GGH, …, HHH) was subjected to alkali titration and pK(a) values were calculated from triplicate potentiometric titrations data using HyperQuad 2008 software. A generalized multiplicative an...

Introduction phate (+pCa+pH,P04) as the function most likely to give a numerical index of the condition in the soil Most of the used for the estimawhich mainly controls the availability of phosphate. tion plant phosphate in are a Its evaluation is jndependent of arbitrary or greater or lesser extent arbitrary, involving the use of procedures. extractants which often have a drastic effect on the...