The string of bacteria, Vibrio. sp. QD-5 utilizing alginate, was separated from rotten kelp. The results of genome sequencing showed that the strain QD-5 contained four alginate lyase genes.One of the alginate genes Aly-IV was cloned and linked to the plasmid pET-22b (+). The heterologous expressed alginate lyase aly-IVwas characterized,which possessed the theoretical molecular mass of 62 kDa, ...

BACKGROUND AND OBJECTIVES Azotobacter vinelandii, a gamma-proteobacterium, is an obligate aerobic free-living gram-negative soil bacterium capable of fixing nitrogen. Oxygen transfer rate into the cell is reduced by the increase of alginate concentrations during the course of A. vinelandii cultivation. This phenomenon provides a low intracellular oxygen concentration needed for nitrogenase acti...

Extracellular alginate lyase secreted by marine Vibrio sp. YWA, isolated from decayed Laminaria japonica, was purified by a combination of ammonium sulfate precipitation and diethylaminoethyl-Sephacel column chromatography. The results show that the molecular mass of alginate lyase was approximately 62.5 kDa, with an optimal pH and temperature at pH 7.0 and 25 degrees C, respectively. K(m) was ...

In chronic respiratory disease, the formation of dense, 3-dimensional "microcolonies" by Pseudomonas aeruginosa within the airway plays an important role in contributing to resistance to treatment. An in vitro biofilm model of pseudomonal microcolony formation using artificial-sputum (AS) medium was established to study the effects of low-molecular-weight alginate oligomers (OligoG CF-5/20) on ...

background: low molecular weight heparin (lmwh) is used for the treatment and prevention of coagulative disorders. few patients receiving therapeutic doses of lmwh develop major hemorrhage. currently there are few reports in the literature on acute overdose on adults.  in this study, clinical profile, treatment and outcome of 21 patients who acutely overdosed enoxaparin are described. methods: ...

Alginate gels are extensively tested in biomedical applications for tissue regeneration and engineering. In this regard, the modification of alginate solutions with dispersed magnetic particles gives extra options to control rheo-elastic properties both fluidic gel forms alginate. Rheological suspensions based on Na-alginate water solution embedded were studied respect interfacial adhesion poly...