given the difficulties in accessing articular cartilage as a source of chondrocytes to be used in fabricating cartilage constructs, alternative sources are required. the present study examined chondrocytes from costal cartilage for their suitability in cartilage tissue engineering. chondrocytes isolated from rat knee and rib hyaline cartilage were separately mixed with alginate and placed in a ...

A new carotenoid-rich product was formed by entrapment of Dunaliella salina in calcium alginate beads of di†erent alginate concentrations, followed by drying in a Ñuid-bed dryer. The drying process yielded b-carotene recoveries of between 79 and 89% and produced a change in the 9-cis/all-trans ratio of b-carotene isomers. The carotenoid stability of the product was dependant on both the storage...

Immobilization of cyanobacterium Spirulina platensis in sodium alginate (1.5 %) gave the best quality of bead and 15-16 beads were formed per mL of aqueous solution of alginate. The immobilized cells were used in a batch process for treatment of diluted sewage. After 8 days, 95 % of BOD5, 77 % of COD, 90 % of ammonia, and 94 % of TSS were removed.

The present work was focused on the development and characterization of new hydrogel systems based on natural origin polymers, namely, alginate and carrageenan, into different formats and with adequate properties to sustain the viability of encapsulated cells, envisioning their application as cell delivery vehicles for tissue regeneration. Different formulations of alginate and carrageenan hydr...

A rapid and simple technique was developed for conjugation between group N and group D streptococci by using cells entrapped within calcium alginate gel beads. With this method, the frequencies of transfer of lactose metabolism from Streptococcus lactis ME2 to S. lactis LM2302 were comparable to those achieved with agar surface matings. Conjugal transfer of the chloramphenicol and erythromycin ...

BACKGROUND In view of compliance with increasingly stringent environmental legislation, an eco-friendly treatment technology of industrial dyes and effluents is a major environmental challenge in the color industry. In present study, a promising and eco-friendly entrapment approach was adopted to immobilize purified manganese peroxidase (MnP) produced from an indigenous strain of Ganoderma luci...

A series of semi-interpenetrating, polymer network (semi-IPN), hydrogel beads, composed of calcium alginate (Ca-alginate) and poly(N-isopropylacrylamide) (PNIPAAM), were prepared for a pH/temperature-sensitive drug delivery study. The equilibrium swelling showed the independent pH- and thermo- responsive nature of the developed materials. At pH=2.1, the release amount of indomethacin incorporat...

Phytate (inositol hexaphosphate; IHP)-degrading microbes have been suggested to contribute to arbuscular mycorrhizal fungi (AMF)-mediated P transfer from IHP to plants; however, no IHP degrader involved in AMF-mediated P transfer has been isolated to date. We herein report the isolation of IHP-degrading bacteria using a modified baiting method. We applied alginate beads as carriers of IHP powde...

Coimmobilization of the freshwater microalga Chlorella vulgaris and the plant-growth-promoting bacterium Azospirillum brasilense in small alginate beads resulted in a significantly increased growth of the microalga. Dry and fresh weight, total number of cells, size of the microalgal clusters (colonies) within the bead, number of microalgal cells per cluster, and the levels of microalgal pigment...

Platelet-rich plasma (PRP) may have the potential to enhance articular cartilage regeneration through release of growth factors including transforming growth factor isoforms. The purpose of this study was to investigate the potential for PRP to stimulate chondrogenic differentiation in three-dimensional PRP hydrogel constructs. Allogenic PRP was prepared using a double centrifugation protocol w...