A plug-based microfluidic approach was used to perform multiple agglutination assays in parallel without cross-contamination and using only microliter volumes of blood. To perform agglutination assays on-chip, a microfluidic device was designed to combine aqueous streams of antibody, buffer, and red blood cells (RBCs) to form droplets 30-40 nL in volume surrounded by a fluorinated carrier fluid...

Leptospirosis is an important global veterinary health problem caused by spirochetes belonging to the genus Leptospira. Leptospires are ubiquitous in nature, and therefore able to adapt to both the ambient environment and the renal tubules of chronically infected reservoir hosts. Microscopic agglutination test (MAT) remains a specialized test, which is not generally performed in the routine dia...

b a ckground: a two year seroepidemiological study was designed to find out the seroprevalence and risk factors ofcanine visceral leishmaniasis (cvl) among stray and owned dogs in tehran and alborz provinces of iran. me t hods: blood samples of  602 dogs living in  11  counties of  tehran and  alborz  provinces were  taken by venipuncture in 2008–2010. after separation of blood sera, anti-leish...

OBJECTIVE To compare the performance of the direct agglutination test and rK39 dipstick for the diagnosis of visceral leishmaniasis. DATA SOURCES Medline, citation tracking, January 1986 to December 2004. Selection criteria Original studies evaluating the direct agglutination test or the rK39 dipstick with clinical visceral leishmaniasis as target condition; adequate reference classification;...

1. The agglutination titer for chicken red cells of freshly prepared or carefully stored suspensions of PR8 influenza virus, that is to say virus of maximum pathogenicity, was found to be proportional to the mouse lethal titer of the same preparations. 2. The agglutination titer of infected allantoic fluid procured in a standard way is relatively constant, regardless of the influenza strain use...

It is shown that when dilute rabbit serum rich in agglutinin for the hog cholera bacillus is heated at 75 degrees C. for 20 minutes and the bacilli incubated with the heated serum, agglutination fails to result on the addition of unheated immune serum. When the immune serum is first heated to 80 degrees C., it no longer greatly inhibits secondary agglutination when the organisms are exposed to ...

Taylor, Neil W. (Northern Regional Research Laboratory, Peoria, Ill.). Inactivation of sexual agglutination in Hansenula wingei and Saccharomyces kluyveri by disulfide-cleaving agents. J. Bacteriol. 88:929-936. 1964.-Mating types of both Hansenula wingei and Saccharomyces kluyveri can be activated to produce uniformly strong sexual agglutination by treatments with various solvents, such as 8 m ...

It has recently been reported that the polycation Polybrene (hexadimethrine bromide), like ristocetin, agglutinates platelets more extensively in the presence of normal plasma than von Willebrand plasma. Since we have previously proposed that ristocetin may initiate agglutination by reducing platelet surface charge, I investigated the correlation between Polybrene's ability to induce agglutinat...

Platelets clump together by two different processes: agglutination and aggregation. Agglutination is a passive process, independent of the metabolic status of the platelets. It is a physical phenomenon of binding of a protein or glycoprotein, which has two or more binding domains. In contrast, platelet aggregation is a metabolically active, transmembrane process that requires “live” platelets. ...