AGF cells were synchronized by blocking the cell cycle at the G1]S boundary with high concentrations of thymidine (thymidine block) for 11 h. Prolongation of the thymidine block from 11 h to 20 h resulted in apoptosis. Early changes in cellular and nuclear morphology were monitored by confocal microscopy, transmission electron microscopy, and scanning electron microscopy. The fluctuations in th...

The unfolding of ribonuclease, lysozyme, a-chymotrypsin, and goat P-lactoglobulin by urea and guanidine hydrochloride (GmHCl) has been followed with the use of optical rotation measurements. Urea denaturation leads to a more negative rotation for each protein than does GmHCl denaturation, but the concentration dependence is such that the rotations are almost identical in the absence of denatura...

Electron attachment to closed-shell molecules is a gateway to various important processes in the gas and condensed phases. The properties of an electron-attached state, such as its energy and lifetime as well as the character of the molecular orbital to which the electron is attached, determine the fate of the anion. In this experimental and theoretical study of copper and silver fluoride anion...

Local and Long-Range Electrostatic Interactions in Denatured Ribonuclease Sa: Comparing the Denatured Protein with Model Peptides (April 2002) Michael Douglas Daily Department of Biochemistry and Biophysics Texas A&M University Fellows Advisor: Dr. J. Martin Scholtz Department of Biochemistry and Biophysics The folding ol'proteins into three-dimensional, biologically active conformations demand...

${\mathrm{AgF}}_{2}$ is a correlated charge-transfer insulator with properties remarkably similar to insulating cuprates, which have raised hope that it may lead new family of unconventional superconductors upon doping. We use ab initio computations study doping strategies leading metallization. Because the upper Hubbard band very narrow electron leads undesired strongly self-trapped states (po...

During a study of the effect,s of certain typical acids, bases and salts upon living planarians (Planaria dorotocephala, P. maculata and P. veEata), observations were made which are here reported on account of the wider interest of their bearing upon tolerance of H+ and OHions, upon the relative efficiency of the mechanism for regulation of neutrality in young and old individuals, and upon the ...