The autoimmobilization of enzymes via cross-linked enzyme crystals (CLECs) has regained interest in recent years, boosted by the extensive knowledge gained protein crystallization, decrease cost and laboriousness process, development potential applications. In this work, we present crystallization preparative-scale production reinforced lipase (RCLLCs) using a commercial detergent additive as r...

Agarose-based gels were produced either by freeze-drying or supercritical drying for crystal violet (CV) removal from aqueous solutions. The microporosity features of these structures highly affected the final adsorption properties. In particular, agarose cryogels characterized a macroporous and irregular morphology, with low value specific surface area (11 ± 6 m2/g) respect to nanoporous aerog...

We present a simple approach for bacterial detection that uses bead-bound RNA-cleaving DNAzyme (RcD) and syringe. The RcD used in this study, F-EC1, is fluorescently labelled can be specifically activated by protein target released E. coli. Upon immobilization on agarose beads, the kinetic behaviour of F-EC1 remains similar to solution. F-EC1-agarose system was able detect as few 1,000 CFUs col...

The most common technique for sorting, identifying, and purifying nucleic acid fragments is agarose gel electrophoresis. performance of the electrophoresis could be impacted by buffers’ composition, ionic strength, pH characteristics [1].

Agarose hydrogels are poroviscoelastic materials that exhibit a waterlogged-crosslinked microstructure. Despite an extensive use in biotechnologies and numerous studies of the elastic properties agarose gels, little is known about compressible behavior microstructural changes such fibrillar under compression. The present work investigates mechanical response centimeter-sized pre-molded cylinder...

Background: Over recent decades, modern protocols of external beam radioÂ‌therapy and radiation techniques such as intensity-modulated radiotherapy (IMRT) have been developed. These methods are extremely sensitive to errors in treatment delivery, so that it is essential to apply a high resolution 3D dosimetry system that has high sensitivity and is capable of measuring and verifying the complex...