Seven laboratories participated in a collaborative study to evaluate the Autobac 1 system. Results obtained with this assay system were compared to those obtained by the standardized Bauer-Kirby disk diffusion test, and each of these two methods was compared to the agar dilution technique. Comparison of the Autobac 1 and the disk diffusion results from the seven laboratories showed an overall a...

abstract- lactic acid bacteria are commonly found in the gastrointestinal tract of various endothermic animals and humans, in milk, dairy and seafood products and on some plant surfaces. the aim of this study was to investigate the inhibitory effect of isolated lactic acid bacteria from intestines of narrow-barred spanish mackerel (scomberomorus commerson) on listeria innocua growth. five strai...

Ampicillin-sulbactam (A/S) and amoxicillin-clavulanic acid (AUG) are thought to be equally efficacious clinically against the Enterobacteriaceae family. In this study, the in vitro activities of the A/S and AUG were evaluated and compared against Escherichia coli and Klebsiella spp. Antimicrobial susceptibility tests were performed by standard agar dilution and disc diffusion techniques accordi...

Intra ocular implants of sodium alginate alone and in combination with hydroxypropylmethylcellulose with or without calcium chloride were formulated with indomethacin as a model drug. The drug release from the implants was evaluated using static method, continuous flow through apparatus (developed in house), USP dissolution and agar diffusion. Except in the static method, indomethacin particle ...

Objective: To investigate the sensitivity pattern of Staphylococcus aureus isolates obtained from clinical specimens including urine, wound, high vaginal swab and semen to commonly used antibiotics. Methodology: The susceptibility patterns of these isolates were determined using the disc diffusion and agar well diffusion methods. Results: Out of 174 samples, 51 (29.2%) yielded S. aureus with th...

Based on the metachromatic property of Toluidine Blue O, three, convenient agar-diffusion methods have been developed that enable detection of the nuclease of Staphylococcus aureus at concentrations as low as 0.005 mug/ml in agar and broth cultures. The interactions of agar and deoxyribonucleic acid with Toluidine Blue O are discussed.

Twelve lots of commercially prepared Mueller-Hinton agar from four medium manufacturers were evaluated for performance with recommended quality control organisms, medium pH, agar depth and uniformity, and characteristics of the zones of inhibition. Only 2 of 12 lots were acceptable for disk diffusion susceptibility tests. Significant problems were observed with the preparation and pouring of th...

Phytochemical and biological investigation of the roots of the wild Daucus carota ssp. carota afforded three new and four known compounds, including four sesquiterpenes daucane esters (1-3 [new], and 4), one polyacetylene (5), one sesquiterpene coumarin (6), and sitosterol glucoside. The structures of the new compounds were determined by comprehensive NMR studies, including DEPT, COSY, NOESY, H...

In vitro interaction of anidulafungin with voriconazole was tested by a microdilution broth checkerboard technique and an agar diffusion method against 30 Aspergillus clinical isolates belonging to five different species. By using a complete inhibition endpoint, indifferent interactions were observed for 97% of the isolates by the checkerboard technique (FIC index from 0.5 to 2) and for 100% of...

To determine appropriate mupirocin susceptibility testing interpretive criteria, 177 staphylococci were tested by agar dilution, disk diffusion, and E test. E test was found to be a reliable method for determining susceptibility of staphylococci to mupirocin. The agar dilution and disk diffusion results were plotted on a scattergram, and error rates were calculated. No errors were found with su...