Isolation and purification of major outer membrane proteins (OMP) from the cell wall envelope of Brucella abortus S-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (Tx-100) and lysozyme treatments, followed by ultracentrifugation. The crude OMP was treated with trypsin to free the preparation from any other protein contaminan...

The rapid growth in demand for peptide drugs is actualizing the search new natural and economically viable sources of raw materials. wide variety biologically active compounds, including nature, inherent marine aquatic organisms allows us to consider their promising material resource. However, use objects as a source production target molecules requires some optimization existing methodological...

The subcritical water (SubCW) extractions of waste wool to produce keratin were performed at temperatures 150 °C 250 and different reaction times between 5 min 75 min. resulting proteins in the obtained products confirmed with Fourier-transform infrared spectroscopy (FTIR). molecular weight protein extracts was determined by using two methods: a polyacrylamide gel electrophoresis presence sodiu...

Microbial manganese peroxidases (MnPs) are ligninolytic enzymes primarily responsible for degrading lignin, but they also capable of dyes, organic pollutants, and emerging contaminants in wastewater. They have been isolated characterized from many white-rot fungi few bacteria. For microbial MnPs, the optimum pH range is between 3.5 9.0; temperature 25°C 70°C. Their molecular weights 25 kDa 68 k...

This study investigated the proteomic difference between juice-producing and cooking EAHBs to clarify role of protein in production banana juice. A comparative was carried out determine content, molecular weight distributions, amino acid profile pulps ten different (five five cooking) cultivars. There low variability crude content cultivars, level fell within range 0.80 g/100 g 1.02 g. SDS-PAGE...