Growth hormone and thyroid hormones have been implicated as important serum factors for adipocyte development in cell culture. Fetal decapitation removes these factors from serum of the growing fetal pig and results in development of fewer adipocytes than in intact fetuses. These experiments examined the effects of growth hormone or thyroxine supplementation to decapitated fetal pig sera upon p...

background: adipose tissue has characteristics of an endocrine organ which releases a number of adipocyte-specific factors, known as adipocytokines.  it has recently suggested that adipocytokines might play a role in pathogenesis and progression of certain cancers, especially in gastric cancer.  this study has managed to investigate endogenous and/or exogenous expression of visfatin and resisti...

context connective tissue cells include fibroblasts, chondrocytes, adipocyte, and osteocytes. these cells are specialized for the secretion of collagenous extracellular matrix and are responsible for the architectural framework of the human body. evidence acquisition connective tissue cells play a central role in supporting as well as repairing tissues and organs. fibroblast cell therapy could ...

We studied the effect of variable isolated fat cell concentrations (from 0.17 to 1.25 x 10(6) cells/ml) on rate and pattern of basal and insulin-stimulated glucose metabolism by rat epididymal fat cells. Cell concentration did not affect total glucose utilization, but high cell concentrations increased the absolute and relative conversion of glucose to CO2 and glyceride-fatty acids by two- to t...

BACK GROUND Intramuscular fat deposition in the meat animal is relatively new strategy for developing the meat quality. Fat deposition is largely depending on the adipocyte proliferation and differentiation. Therefore, we investigated the effect of chloroform extract of L. multiflorum [CELM] on cell proliferation, lipid accumulation and adipocyte differentiation in 3T3-L1 cells and body weight ...

Adipocyte is closely related to energy homeostasis. While white adipocyte stores energy as a form of lipids, brown adipocyte dissipates energy by producing heat. In mice and humans exposed to chronic cold temperature, white adipocyte transdifferentiates into brown adipocyte-like cell called beige (brite) cell. The regulation of brown adipocyte function and beigeing of white adipocyte is conside...

Adipose tissue cellularity and metabolism are traditionally expressed in terms of mean cell size and number. The need for a simple method allowing rapid determination of cell size and number of freshly isolated, unfixed adipocyte preparations led us to compare estimates of cell size determined by the established method of optical sizing to a proposed method of electronic cell sizing and countin...

Expression of bone morphogenetic protein 4 (BMP4) in adipocytes of white adipose tissue (WAT) produces "white adipocytes" with characteristics of brown fat and leads to a reduction of adiposity and its metabolic complications. Although BMP4 is known to induce commitment of pluripotent stem cells to the adipocyte lineage by producing cells that possess the characteristics of preadipocytes, its e...

In this study, we explored the roles of microRNAs in adipocyte differentiation and metabolism. We first knocked down Argonaute2 (Ago2), a key enzyme in the processing of micro-RNAs (miRNAs), to investigate a potential role for miRNAs in adipocyte differentiation and/or metabolism. Although we did not observe dramatic differences in adipogenesis between Ago2 knock-down and control 3T3-L1 cells, ...

background: human dental stem cells have high proliferative potential for self-renewal that is important to the regenerative capacity of the tissue. objective: the aim was to isolate human dental pulp stem cells (dpsc), periodontal ligament stem cells (pdlsc) and periapical follicle stem cells (pafsc) for their potential role in tissue regeneration. materials and methods: in this experimental s...