The present study was carried out with the following objectives: (1) to isolate the endophytic bacilli strains from the leaves of mangrove plant Avicennia marina, (2) to screen the potential strains for the production of adenosine deaminase, (3) to statistically optimize the factors that influence the enzyme activity in the potent strain, and (4) to identify the potent strain using 16S rRNA seq...

Myocardial ischemia is characterized by the liberation of adenosine and by complement-mediated inflammation. We have reported that amidated C3, formed when ammonia (NH3) disrupts the thiolester bond of C3, serves as an alternative pathway convertase, generates C5b-9, and stimulates phagocytic oxidative metabolism. We investigated whether the deamination of adenosine by adenosine deaminase in he...

Senescent erythrocytes from rabbits were previously shown to have elevated levels of adenine nucleotides. The present study documents that aged red blood cells have a normal synthetic capacity for adenine nucleotides, as indicated by normal levels of adenosine kinase. However, senescent erythrocytes do have decreased levels of adenosine 5'-monophosphate deaminase, the critical enzyme involved i...

Pendahuluan. Diagnosis tuberkulosis (TB) pada anak merupakan masalah tersendiri karena manifestasi klinis yang beragam dan tidak tersedianya standar baku penegakan diagnosis. Kasus. Anak perempuan usia 6 tahun, dengan demam, batuk, sesak napas. Pada pemeriksaan fisik didapatkan penurunan suara napas di rongga dada kanan. Hasil radiologi didukung ultrasonografi tampak gambaran efusi pleura kanan...

To test whether extracellular adenosine participates in the local regulation of intestinal blood flow during nutrient absorption, the serosa of the jejunum was continuously suffused with adenosine deaminase (7 micrograms protein/ml) or theophylline (10(-4) M) in Ringer's solution. Using video microscopy, blood flow was calculated in submucosal arterioles from diameter and red cell velocity meas...

Pathways producing and converting adenosine have hardly been investigated in human heart, contrasting work in other species. We compared the kinetics of enzymes associated with purine degradation and salvage in human and rat heart cytoplasm assaying for adenosine deaminase, nucleoside phosphorylase, xanthine oxidoreductase, AMP deaminase, AMP- and IMP-specific 5'-nucleotidases, adenosine kinase...

Deoxyadenosine 5’-triphosphate and deoxyadenosine 5’diphosphate are the most abundant nucleotides in erythrocytes of an untreated child with adenosine deaminase deficiency and severe combined immunodeficiency disease. Since ATP and ADP are the predominant nucleotides in normal erythrocytes and the deoxynucleotides are virtually absent, the high levels of dATP and dADP represent a major perturba...

The double-stranded RNA (dsRNA) adenosine deaminase (DRADA) deaminates adenosine residues to inosines and creates I-U mismatched base pairs in dsRNAs. Its involvement in RNA editing of glutamate-gated ion channel gene transcripts in mammalian brains has been proposed as one of the biological functions for this recently identified cellular enzyme. We purified a mixture of three forms, 93, 88, an...

Adenosine synthesis was studied during 2-deoxyglucose-induced ATP catabolism in intact rat polymorphonuclear leucocytes. When both adenosine kinase (EC 2.7.1.20) and adenosine deaminase (EC 3.5.4.4) were selectively inhibited, adenosine accumulated. Adenosine formation took place inside the intact cells by a metabolic pathway independent of the ecto-5'-nucleotidase (EC 3.1.3.5). Distinct metabo...

BACKGROUND Adenine and adenosine-acting aminohydrolases are important groups of enzymes responsible for the metabolic salvage of purine compounds. Several subclasses of these enzymes have been described and given current knowledge of the full genome sequences of many organisms, it is possible to identify genes encoding these enzymes and group them according to their primary structure. METHODS...